The correlation between extracellular heat shock protein 70 and lipid metabolism in a ruminant model

Petrović, Miloš Ž. (author), Cincović, M. R. (author), Starič, Jože (author), Djoković, Radojica (author), Belić, B. (author), Radinović, Miodrag (author), Majkić, Mira (author), Ilić, Zoran Ž. (author)

Metabolic stress in early lactation cows is characterized by lipolysis, ketogenesis, insulin resistance and inflammation because of negative energy balance and increased use of lipids for energy needs. In this study the relationship between lipid metabolite, lipid-based insulin resistance, and hepatocyte functionality indexes and tumor necrosis factor alpha (TNF-α) with extracellular heat shock protein 70 (eHsp70) was investigated. The experiment included 50 cows and all parameters were measured in blood serum. In cows with a more pronounced negative energy balance, the following was determined: a higher concentration of eHsp70, TNF-α, non-esterified fatty acid (NEFA), beta-hydroxybutyrate (BHB), NEFA to insulin and NEFA to cholesterol ratio and lower concentration of cholesterol, very low-density lipoproteins (VLDL), low density lipoproteins (LDL) and liver functionality index (LFI). The eHsp70 correlated negatively with the values of cholesterol, VLDL, LDL, and triglycerides, while correlated positively with the level of NEFA and BHB. A higher concentration of eHsp70 suggests the development of fatty liver (due to a higher NEFA to cholesterol ratio and lower LFI) and insulin resistance (due to a lower revised quantitative insulin sensitivity check index RQUICKI-BHB and higher NEFA to insulin ratio). The eHsp70 correlated positively with TNF-α. Both TNF-α and eHsp70 correlated similarly to lipid metabolites. In cows with high eHsp70 and TNF-α values we found higher concentrations of NEFA, BHB, NEFA to insulin and NEFA to cholesterol ratio and a lower concentration of triglycerides and VLDL cholesterol compared to cows that had only high TNF-α values. Based on the positive correlation between eHsp70 and TNF-α, their similar relations, and the additional effect of eHsp70 (high TNF-α + eHsp70 values) on lipid metabolites we conclude that eHsp70 has pro-inflammatory effects implicating lipolysis, fatty liver, and fat tissue insulin resistance.

• Language: English
• Keywords: cow, early lactation, chaperons, lipolysis, ketogensis, insulin resistance, fatty liver, negative energy balance, cattle, lactation
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2022
• Number of pages: 14 str.
• Numbering: Vol. 12, iss. 1, art. 19
• PID: 20.500.12556/RUL-134220
• UDC: 636.09:542
• ISSN on article: 2079-6382
• DOI: 10.3390/metabo12010019
• COBISS.SI-ID: 91366915
• Publication date in RUL: 30.12.2021

Record is a part of a journal

• Title: Metabolites
• Shortened title: Metabolites
• Publisher: MDPI AG
• ISSN: 2218-1989
• COBISS.SI-ID: 523274009

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 01.01.2022

Projects

• Funder: Other - Other funder or multiple funders
• Funding programme: Ministry of Education, Science and Technological Development
• Project number: 451-03-9/2021-14/200117