Adhesion of Campylobacter jejuni is increased in association with foodborne bacteria

Klančnik, Anja (author), Gobin, Ivana (author), Jeršek, Barbara (author), Smole Možina, Sonja (author), Vučković, Darinka (author), Tušek-Žnidarič, Magda (author), Abram, Maja (author)

The aim of this study was to evaluate Campylobacter jejuni NTCT 11168 adhesion to abiotic and biotic surfaces when grown in co-culture with Escherichia coli ATCC 11229 and/or Listeria monocytogenes 4b. Adhesion of C. jejuni to polystyrene and to Caco-2 cells and Acanthamoeba castellanii was lower for at least 3 log CFU/mL compared to E. coli and L. monocytogenes. Electron micrographs of ultrathin sections revealed interactions of C. jejuni with host cells. In co-culture with E. coli and L. monocytogenes, adhesion of C. jejuni to all tested surfaces was significantly increased for more than 1 log CFU/mL. There was 10% higher aggregation for C. jejuni than for other pathogens, and high co-aggregation of co-cultures of C. jejuni with E. coli and L. monocytogenes. These data show that C. jejuni in co-cultures with E. coli and L. monocytogenes present significantly higher risk than C. jejuni as mono-cultures, which need to be taken into account in risk evaluation. C. jejuni adhesion is a prerequisite for their colonization, biofilm formation, and further contamination of the environment. C. jejuni survival under adverse conditions as a factor in their pathogenicity and depends on their adhesion to different surfaces, not only as individual strains, but also in co-cultures with other bacteria like E. coli and L. monocytogenes.

• Language: English
• Keywords: adhesion, abiotic/biotic surface, Campylobacter jejuni, co-culture, survival, pathogenicity
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2020
• Number of pages: 14 str.
• Numbering: Vol. 8, iss. 2, art. 201
• PID: 20.500.12556/RUL-133219
• UDC: 579.67:579.26:547.9
• ISSN on article: 2076-2607
• DOI: 10.3390/microorganisms8020201
• COBISS.SI-ID: 5171320
• Publication date in RUL: 17.11.2021

Record is a part of a journal

• Title: Microorganisms
• Shortened title: Microorganisms
• Publisher: MDPI AG
• ISSN: 2076-2607
• COBISS.SI-ID: 523277081

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 01.02.2020

Secondary language

• Language: Slovenian
• Keywords: patogeni mikroorganizmi, Campylobacter jejuni, bakterijska adhezija, bakterijski biofilmi, celična adhezija, polistiren