Viscosity of plasma as a key factor in assessment of extracellular vesicles by light scattering

Božič, Darja (author), Sitar, Simona (author), Junkar, Ita (author), Štukelj, Roman (author), Pajnič, Manca (author), Žagar, Ema (author), Kralj-Iglič, Veronika (author), Kogej, Ksenija (author)

Extracellular vesicles (EVs) isolated from biological samples are a promising material for use in medicine and technology. However, the assessment methods that would yield repeatable concentrations, sizes and compositions of the harvested material are missing. A plausible model for the description of EV isolates has not been developed. Furthermore, the identity and genesis of EVs are still obscure and the relevant parameters have not yet been identified. The purpose of this work is to better understand the mechanisms taking place during harvesting of EVs, in particular the role of viscosity of EV suspension. The EVs were harvested from blood plasma by repeated centrifugation and washing of samples. Their size and shape were assessed by using a combination of static and dynamic light scattering. The average shape parameter of the assessed particles was found to be ρ ~ 1 (0.94–1.1 in exosome standards and 0.7–1.2 in blood plasma and EV isolates), pertaining to spherical shells (spherical vesicles). This study has estimated the value of the viscosity coefficient of the medium in blood plasma to be 1.2 mPa/s. It can be concluded that light scattering could be a plausible method for the assessment of EVs upon considering that EVs are a dynamic material with a transient identity.

• Language: English
• Keywords: extracellular vesicles, exosomes, blood plasma, dynamic light scattering, static light scattering, shape characterization, viscosity of blood plasma
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FKKT - Faculty of Chemistry and Chemical Technology; ZF - Faculty of Health Sciences
• Publication status: Published
• Publication version: Version of Record
• Year: 2019
• Number of pages: 21 str.
• Numbering: Vol. 8, iss. 9, art. 1046
• PID: 20.500.12556/RUL-132807
• UDC: 577.35
• ISSN on article: 2073-4409
• DOI: 10.3390/cells8091046
• COBISS.SI-ID: 1538312131
• Publication date in RUL: 03.11.2021

Record is a part of a journal

• Title: Cells
• Shortened title: Cells
• Publisher: MDPI
• ISSN: 2073-4409
• COBISS.SI-ID: 519958809

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 06.09.2019

Secondary language

• Language: Slovenian
• Keywords: izvencelični vezikli, eksosomi, krvna plazma, dinamično sipanje svetlobe, statično sipanje svetlobe, določanje oblike, viskoznost krvne plazme