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Viscosity of plasma as a key factor in assessment of extracellular vesicles by light scattering
ID
Božič, Darja
(
Author
),
ID
Sitar, Simona
(
Author
),
ID
Junkar, Ita
(
Author
),
ID
Štukelj, Roman
(
Author
),
ID
Pajnič, Manca
(
Author
),
ID
Žagar, Ema
(
Author
),
ID
Kralj-Iglič, Veronika
(
Author
),
ID
Kogej, Ksenija
(
Author
)
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MD5: 05179C0E1F0114206C00B5056480E4ED
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https://www.mdpi.com/2073-4409/8/9/1046
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Abstract
Extracellular vesicles (EVs) isolated from biological samples are a promising material for use in medicine and technology. However, the assessment methods that would yield repeatable concentrations, sizes and compositions of the harvested material are missing. A plausible model for the description of EV isolates has not been developed. Furthermore, the identity and genesis of EVs are still obscure and the relevant parameters have not yet been identified. The purpose of this work is to better understand the mechanisms taking place during harvesting of EVs, in particular the role of viscosity of EV suspension. The EVs were harvested from blood plasma by repeated centrifugation and washing of samples. Their size and shape were assessed by using a combination of static and dynamic light scattering. The average shape parameter of the assessed particles was found to be ρ ~ 1 (0.94–1.1 in exosome standards and 0.7–1.2 in blood plasma and EV isolates), pertaining to spherical shells (spherical vesicles). This study has estimated the value of the viscosity coefficient of the medium in blood plasma to be 1.2 mPa/s. It can be concluded that light scattering could be a plausible method for the assessment of EVs upon considering that EVs are a dynamic material with a transient identity.
Language:
English
Keywords:
extracellular vesicles
,
exosomes
,
blood plasma
,
dynamic light scattering
,
static light scattering
,
shape characterization
,
viscosity of blood plasma
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
FKKT - Faculty of Chemistry and Chemical Technology
ZF - Faculty of Health Sciences
Publication status:
Published
Publication version:
Version of Record
Year:
2019
Number of pages:
21 str.
Numbering:
Vol. 8, iss. 9, art. 1046
PID:
20.500.12556/RUL-132807
UDC:
577.35
ISSN on article:
2073-4409
DOI:
10.3390/cells8091046
COBISS.SI-ID:
1538312131
Publication date in RUL:
03.11.2021
Views:
938
Downloads:
187
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Record is a part of a journal
Title:
Cells
Shortened title:
Cells
Publisher:
MDPI
ISSN:
2073-4409
COBISS.SI-ID:
519958809
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:
06.09.2019
Secondary language
Language:
Slovenian
Keywords:
izvencelični vezikli
,
eksosomi
,
krvna plazma
,
dinamično sipanje svetlobe
,
statično sipanje svetlobe
,
določanje oblike
,
viskoznost krvne plazme
Projects
Funder:
ARRS - Slovenian Research Agency
Project number:
P1-0201
Name:
Fizikalna kemija
Funder:
ARRS - Slovenian Research Agency
Project number:
P2-0145
Name:
Polimeri in polimerni materiali s posebnimi lastnostmi
Funder:
ARRS - Slovenian Research Agency
Project number:
P3-0388
Name:
Mehanizmi varovanja zdravja
Funder:
ARRS - Slovenian Research Agency
Project number:
L7-7566
Name:
Napredne hemokompatibilne površine žilnih opornic
Funder:
ARRS - Slovenian Research Agency
Project number:
J2-8166
Name:
Anizotropni magnetni nanodelci za magneto-mehansko zdravljenje raka
Funder:
ARRS - Slovenian Research Agency
Project number:
J1-9162
Name:
Neurotoksičnost ali neuroprotektivnost nanomaterialov: vpliv biokorone
Funder:
ARRS - Slovenian Research Agency
Project number:
J2-8169
Name:
Piezoelektrični biomateriali za regeneracijo s pomočjo elektro-stimulacije
Funder:
EC - European Commission
Funding programme:
H2020
Project number:
801338
Name:
Extracellular vesicles from a natural source for tailor-made nanomaterials
Acronym:
VES4US
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