THP-1 cells and pro-inflammatory cytokine production : an in vitro tool for functional characterization of NOD1/NOD2 antagonists

Jakopin, Žiga (author), Corsini, Emanuela (author)

THP-1 cells express high levels of native functional nucleotide-binding oligomerization domain 1 (NOD1), NOD2, and Toll-like receptor 4 (TLR4) receptors, and have often been used for investigating the immunomodulatory effects of small molecules. We postulated that they would represent an ideal cell-based model for our study, the aim of which was to develop a new in vitro tool for functional characterization of NOD antagonists. NOD antagonists were initially screened for their effect on NOD agonist-induced interleukin-8 (IL-8) release. Next, we examined the extent to which the selected NOD antagonists block the NOD-TLR4 synergistic crosstalk by measuring the effect of NOD antagonism on tumor necrosis factor-α (TNF-α) secretion from doubly activated THP-1 cells. Overall, the results obtained indicate that pro-inflammatory cytokine secretion from THP-1 provides a valuable, simple and reproducible in vitro tool for functional characterization of NOD antagonists.

• Language: English
• Keywords: THP-1, cytokines, IL-8, TNF-α, immunomodulation, NOD1 antagonist, NOD2 antagonist, functional characterization
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FFA - Faculty of Pharmacy
• Publication status: Published
• Publication version: Version of Record
• Year: 2019
• Number of pages: 11 str.
• Numbering: Vol. 20, iss. 17, art. 4265
• PID: 20.500.12556/RUL-132772
• UDC: 615.375:61
• ISSN on article: 1422-0067
• DOI: 10.3390/ijms20174265
• COBISS.SI-ID: 4770417
• Publication date in RUL: 03.11.2021

Record is a part of a journal

• Title: International journal of molecular sciences
• Shortened title: Int. j. mol. sci.
• Publisher: MDPI
• ISSN: 1422-0067
• COBISS.SI-ID: 2779162

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 01.09.2019

Secondary language

• Language: Slovenian
• Keywords: citokini, imunomodulatorji

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P1-0208
• Name: Farmacevtska kemija: načrtovanje, sinteza in vrednotenje učinkovin

• Funder: Other - Other funder or multiple funders
• Funding programme: COST
• Project number: CA15135
• Name: Multi-target paradigm for innovative ligand identification in the drug discovery process
• Acronym: MuTaLig