THP-1 cells and pro-inflammatory cytokine production : an in vitro tool for functional characterization of NOD1/NOD2 antagonists
ID Jakopin, Žiga (Author), ID Corsini, Emanuela (Author)

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THP-1 cells express high levels of native functional nucleotide-binding oligomerization domain 1 (NOD1), NOD2, and Toll-like receptor 4 (TLR4) receptors, and have often been used for investigating the immunomodulatory effects of small molecules. We postulated that they would represent an ideal cell-based model for our study, the aim of which was to develop a new in vitro tool for functional characterization of NOD antagonists. NOD antagonists were initially screened for their effect on NOD agonist-induced interleukin-8 (IL-8) release. Next, we examined the extent to which the selected NOD antagonists block the NOD-TLR4 synergistic crosstalk by measuring the effect of NOD antagonism on tumor necrosis factor-α (TNF-α) secretion from doubly activated THP-1 cells. Overall, the results obtained indicate that pro-inflammatory cytokine secretion from THP-1 provides a valuable, simple and reproducible in vitro tool for functional characterization of NOD antagonists.

Keywords:THP-1, cytokines, IL-8, TNF-α, immunomodulation, NOD1 antagonist, NOD2 antagonist, functional characterization
Work type:Article (dk_c)
Typology:1.01 - Original Scientific Article
Organization:FFA - Faculty of Pharmacy
Publication status in journal:Published
Article version:Publisher's version of article
Number of pages:11 str.
Numbering:Vol. 20, iss. 17, art. 4265
ISSN on article:1422-0067
DOI:10.3390/ijms20174265 This link opens in a new window
COBISS.SI-ID:4770417 This link opens in a new window
Publication date in RUL:03.11.2021
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Record is a part of a journal

Title:International journal of molecular sciences
Shortened title:Int. j. mol. sci.
COBISS.SI-ID:2779162 This link opens in a new window


License:CC BY 4.0, Creative Commons Attribution 4.0 International
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:01.09.2019

Secondary language

Keywords:citokini, imunomodulatorji

Document is financed by a project

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Name:Farmacevtska kemija: načrtovanje, sinteza in vrednotenje učinkovin

Funder:Drugi - Drug financer ali več financerjev
Funding Programme:COST
Name:Multi-target paradigm for innovative ligand identification in the drug discovery process

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