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Vpliv hladne atmosferske plazme na virus blage lisavosti paprike : magistrsko delo
ID Škripec, Uroš (Author), ID Žel, Jana (Mentor) More about this mentor... This link opens in a new window, ID Vogel-Mikuš, Katarina (Member of the commission for defense), ID Dolenc Koce, Jasna (Reviewer)

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Abstract
V naši raziskovalni nalogi smo preučevali vpliv hladne atmosferske plazme na virus blage lisavosti paprike (PMMoV; angl. pepper mild mottle virus). Želeli smo ugotoviti, če je hladna atmosferska plazma primerna metoda za inaktivacijo PMMoV v vodi. PMMoV je rastlinski patogen, ki se v odpadnih vodah nahaja v visokih koncentracijah in ga je s tradicionalnimi metodami za čiščenje vode težko inaktivirati. Poleg tega imajo lahko tradicionalne metode določene negativne lastnosti kot so visoka cena in nastanek škodljivih produktov. Po obdelavi vzorcev vode, ki je vsebovala PMMoV, s hladno atmosfersko plazmo, smo na testnih rastlinah izvedli teste infektivnosti, prisotnost PMMoV v testnih rastlinah pa smo preverili z verižno reakcijo s polimerazo z reverzno transkripcijo v realnem času. Vpliv hladne atmosferske plazme na virusno RNK oz. njeno razgradnjo smo preverili z verižno reakcijo s polimerazo z reverzno transkripcijo, namnožene produkte pa smo določali z gelsko elektroforezo. Pokazali smo, da je PMMoV mogoče inaktivirati že po pet minutni obdelavi s hladno atmosfersko plazmo, v tem času pa je prišlo tudi do razgradnje RNK. Zaradi omenjenega, bi hladna atmosferska plazma v prihodnosti lahko predstavljala pomembno orodje za čiščenje odpadnih voda in inaktivacijo prisotnih patogenov.

Language:Slovenian
Keywords:Rastlinski virusi, tobamovirusi, virus blage lisavosti paprike, hladna atmosferska plazma, dekontaminacija vode, verižna reakcija s polimerazo
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Place of publishing:Ljubljana
Publisher:[U. Škripec]
Year:2021
Number of pages:X f., 39 str.
PID:20.500.12556/RUL-132520 This link opens in a new window
UDC:577.2(043.2)
COBISS.SI-ID:83347203 This link opens in a new window
Publication date in RUL:28.10.2021
Views:2021
Downloads:83
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Secondary language

Language:English
Title:The effect of cold atmospheric plasma on pepper mild mottle virus. : magistrski študij - 2. stopnja
Abstract:
In our research, we investigated the effect of cold atmospheric plasma on pepper mild mottle virus (PMMoV). The aim was to find out if cold atmospheric plasma is a suitable method to inactivate PMMoV in water. PMMoV is a plant pathogen that is found in high concentrations in wastewater and is difficult to inactivate using conventional water treatment methods. Moreover, these methods may have some limitations, such as high cost and the formation of harmful by-products. After treating water samples containing PMMoV with cold atmospheric plasma, infectivity assays were performed on test plants. The presence of PMMoV in the test plants was determined by reverse transcription real-time polymerase chain reaction. The effect of cold atmospheric plasma on viral RNA degradation was determined by reverse-transcription polymerase chain reaction, and the amplified products were detected by gel electrophoresis. We have shown that it is possible to inactivate PMMoV after only 5 min of treatment with cold atmospheric plasma, during which time RNA was also degraded. Therefore, cold atmospheric plasma could be an important tool for wastewater treatment and inactivation of existing pathogens in the future.

Keywords:Plant viruses, tobamoviruses, pepper mild mottle virus, cold atmospheric pressure plasma, water decontamination, polymerase chain reaction

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