Sexual dimorphism of the extraorbital lacrimal glands in SF-1 knockout mice
ID Šemanjski, Kristina (Author), ID Majdič, Gregor (Author), ID Kozina, Viviana (Author), ID Ježek, Davor (Author)

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ABSTRACT Sexual dimorphism (SD) represents all the differences between males and females of the same species. SD of the murine lacrimal gland and the major effect of testosterone on its formation are well documented. Steroidogenic factor-1 (SF-1, NR5a1) is a nuclear receptor essential for the fetal development of steroid hormones producing organs and SF-1 knockout mice (Sf-1 KO) are therefore born without gonads and adrenal glands. The aim of this study was to investigate whether SD in lacrimal glands is present in the absence of exposure to sex hormones during development. Lacrimal glands from adult Sf-1 KO male and female mice without hormonal exposure, and from males that were treated with testosterone propionate (TP) prior to sacrifice, were examined. After sacrifice, glandular tissue was processed using standard histological procedures. Paraffin sections were analysed by ste-reology and immunostained against the androgen receptor (AR). Our results showed that there were no statis-tically significant differences in the mean volumes of acini, connective tissue or ductal system between males, females, and males on TP. The same pertains to the mean length of the ducts in all three groups. In the absence of sex hormones, sex chromosomes proved to be insufficient in inducing sexual dimorphism in LG. However, nuclei of the acinar cells in males on TP were positive for AR, whereas in males without TP no expression of AR was detected. Administration of TP induced the expression of AR in the nuclei of acinar cells of males but did not affect the morphology of LG. We conclude that SD in the lacrimal gland is not present in Sf-1 KO mice and this suggests that sex hormones have a major role in the development of SD in the lacrimal gland.

Keywords:Sexual dimorphism, lacrimal gland, testosterone, Sf-1 KO mice
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:VF - Veterinary Faculty
Publication status:Published
Publication version:Author Accepted Manuscript
Publication date:25.12.2020
Number of pages:7 str.
Numbering:Vol. 123, no. 2, art. 151669
PID:20.500.12556/RUL-132237 This link opens in a new window
ISSN on article:1618-0372
DOI:10.1016/j.acthis.2020.151669 This link opens in a new window
COBISS.SI-ID:46732035 This link opens in a new window
Publication date in RUL:18.10.2021
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Record is a part of a journal

Title:Acta histochemica
Shortened title:Acta histochem.
COBISS.SI-ID:46718211 This link opens in a new window


License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:25.12.2020


Funder:MZOS - Ministry of Science, Education and Sports of the Republic of Croatia
Project number:108-1080399-0383
Name:Male and female reproductive tract: development, histophysiology and infertility

Funder:EC - European Commission
Funding programme:European Union through the European Regional Development Fund
Project number:KK.
Name:Regenerative and Reproductive Medicine - Exploring New Platforms and Potentials

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