Results of dynamic contrast-enhanced ultrasound correlate with treatment outcome in canine neoplasia treated with electrochemotherapy and interleukin-12 plasmid electrotransfer

Brložnik, Maja (author), Kranjc Brezar, Simona (author), Boc, Nina (author), Knific, Tanja (author), Čemažar, Maja (author), Milevoj, Nina (author), Serša, Gregor (author), Tozon, Nataša (author), Pavlin, Darja (author)

Electrochemotherapy (ECT) and/or gene electrotransfer of plasmid DNA encoding interleukin-12 (GET pIL-12) are effective treatments for canine cutaneous, subcutaneous, and maxillofacial tumors. Despite the clinical efficacy of the combined treatments of ECT and GET, data on parameters that might predict the outcome of the treatments are still lacking. This study aimed to investigate whether dynamic contrast-enhanced ultrasound (DCE-US) results of subcutaneous tumors differ between tumors with complete response (CR) and tumors without complete response (non-CR) in dogs treated with ECT and GET pIL-12. Eight dogs with a total of 12 tumor nodules treated with ECT and GET pIL-12 were included. DCE-US examinations were performed in all animals before and immediately after therapy as well as 8 h and 1, 3, and 7 days later. Clinical follow-up examinations were performed 7 and 14 days, 1 and 6 months, and 1 year after treatment. Numerous significant differences in DCE-US parameters were noted between tumors with CR and non-CR tumors; perfusion and perfusion heterogeneity were lower in CR tumors than in non-CR tumors. Therefore, studies with larger numbers of patients are needed to investigate whether DCE-US results can be used to predict treatment outcomes and to make effective decisions about the need for repeated therapy or different treatment combinations in individual patients.

• Language: English
• Keywords: dog, contrast-enhanced ultrasonography, electrochemotherapy, immunotherapy, gene electrotransfer, bleomycin, cisplatin, IL-12 plasmid
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty; MF - Faculty of Medicine; ZF - Faculty of Health Sciences
• Publication status: Published
• Publication version: Version of Record
• Submitted for review: 10.03.2021
• Article acceptance date: 09.04.2021
• Publication date: 20.05.2021
• Year: 2021
• Number of pages: 12 str
• Numbering: Vol. 8, art. 679073
• PID: 20.500.12556/RUL-132092
• UDC: 636.09:616
• ISSN on article: 2297-1769
• DOI: 10.3389/fvets.2021.679073
• COBISS.SI-ID: 63974403
• Publication date in RUL: 12.10.2021

Record is a part of a journal

• Title: Frontiers in veterinary science
• Shortened title: Front. vet. sci.
• Publisher: Frontiers Media
• ISSN: 2297-1769
• COBISS.SI-ID: 3969402

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 20.05.2021