Genetic characterisation and comparison of three human coronaviruses (HKU1, OC43, 229E) from patients and bovine coronavirus (BCoV) from cattle with respiratory disease in Slovenia

Jevšnik Virant, Monika (author), Černe, Danijela (author), Petrovec, Miroslav (author), Paller, Tomislav (author), Toplak, Ivan (author)

Coronaviruses (CoV) are widely distributed pathogens of human and animals and can cause mild or severe respiratory and gastrointestinal disease. Antigenic and genetic similarity of some CoVs within the Betacoronavirus genus is evident. Therefore, for the first time in Slovenia, we investigated the genetic diversity of partial 390-nucleotides of RNA-dependent-RNA polymerase gene (RdRp) for 66 human (HCoV) and 24 bovine CoV (BCoV) positive samples, collected between 2010 and 2016 from human patients and cattle with respiratory disease. The characterized CoV strains belong to four different clusters, in three separate human clusters HCoV-HKU1 (n = 34), HCoV-OC43 (n = 31) and HCoV 229E (n = 1) and bovine grouping only as BCoVs (n = 24). BCoVs from cattle and HCoV-OC43 were genetically the most closely related and share 96.4–97.1% nucleotide and 96.9–98.5% amino acid identity.

• Language: English
• Keywords: coronaviruses, genetic diversity, HCoV-OC43, BCoV, transmission, genetic variation, human coronavirus OC43
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: MF - Faculty of Medicine; VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: 10 str.
• Numbering: Vol. 13, iss. 4, art. 676
• PID: 20.500.12556/RUL-132086
• UDC: 616
• ISSN on article: 1999-4915
• DOI: 10.3390/v13040676
• COBISS.SI-ID: 59800835
• Publication date in RUL: 12.10.2021

Record is a part of a journal

• Title: Viruses
• Shortened title: Viruses
• Publisher: MDPI
• ISSN: 1999-4915
• COBISS.SI-ID: 517597977

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 15.04.2021

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P3-0083
• Name: Odnosi parazitskega obstajanja

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0092
• Name: Zdravje živali, okolje in varna hrana