Antibiofilm potential of Lavandula preparations against Campylobacter jejuni

Ramić, Dina (author), Bucar, Franz (author), Kunej, Urban (author), Dogša, Iztok (author), Klančnik, Anja (author), Smole Možina, Sonja (author)

New approaches for the control of Campylobacter jejuni biofilms in the food industry are being studied intensively. Natural products are promising alternative antimicrobial substances to control biofilm production, with particular emphasis on plant extracts. Dried flowers of Lavandula angustifolia were used to produce LEO, LEF, and LEW. The chemical compositions determined for these Lavandula preparations included seven major compounds that were selected for further testing. These were tested against C. jejuni, for biofilm degradation and removal. Next-generation sequencing was used to study the molecular mechanisms underlying LEO actions against C. jejuni adhesion and motility. Analysis of LEO revealed 1,8-cineol, linalool and linalyl acetate as the main components. For LEF and LEW, the main components were phenolic acid glycosides, with flavonoids rarely present. The minimal inhibitory concentrations of the Lavandula preparations and pure compounds against C. jejuni ranged from 0.2 mg/mL to 1 mg/mL. LEO showed the strongest biofilm degradation. The reduction of C. jejuni adhesion was ≥1 log10 CFU/mL, which satisfies European Food Safety Authority recommendations. Lavandula preparations reduced C. jejuni motility by almost 50%, which consequently can impact upon biofilm formation. These data are in line with the transcriptome analysis of C. jejuni, which indicated that LEO down-regulated genes important for biofilm formation. LEW also showed good antibacterial and anti-biofilm effects, particularly against adhesion and motility mechanisms. This defines an innovative approach using alternative strategies and novel targets to combat bacterial biofilm formation, and hence the potential to develop new effective agents with biofilm-degrading activities.

• Language: English
• Keywords: Lavandula preparations, antibiofilm activity, Campylobacter jejuni
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Author Accepted Manuscript
• Publisher: American Society for Microbiology
• Year: 2021
• Number of pages: 18 str.
• Numbering: Vol. 87, iss. 19, art. e01099-21
• PID: 20.500.12556/RUL-131821
• UDC: 579.24/.26:547.9
• ISSN on article: 1098-5336
• DOI: 10.1128/AEM.01099-21
• COBISS.SI-ID: 72216835
• Publication date in RUL: 04.10.2021

Record is a part of a journal

• Title: Applied and environmental microbiology
• Shortened title: Appl. environ. microbiol.
• Publisher: American Society for Microbiology
• ISSN: 1098-5336
• COBISS.SI-ID: 2926612

Secondary language

• Language: Slovenian
• Keywords: rastlinski izvlečki, protimikrobne snovi, sivka, Campylobacter jejuni, biofilm