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Bioaktivne lastnosti ekstraktov mikroalg vrst Arthrospira platensis in Chlorella vulgaris
Grum, Barbara (Author), Jeršek, Barbka (Mentor) More about this mentor... This link opens in a new window

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Abstract
Mikroalge so zaradi sposobnosti proizvajanja širokega spektra organskih spojin, med katerimi imajo številne bioaktivne lastnosti, vse bolj proučevane na različnih znanstvenih področjih. Med temi spojinami so še posebej zanimive tiste s protimikrobnim in protiadhezivnim delovanjem. V raziskavi smo proučevali bioaktivne lastnosti mikroalg vrst Arthrospira platensis in Chlorella vulgaris pri bakterijah vrst Escherichia coli, Pseudomonas aeruginosa ter Staphylococcus aureus in kvasovkah vrste Candida albicans. Določali smo protimikrobno delovanje z metodo razredčevanja v mikrotitrski plošči, s katero smo izbranim ekstraktom mikroalg določili minimalno inhibitorno koncentracijo (MIK) ter protiadhezivno delovanje z metodo barvanja s kristal vijoličnim, za kar smo uporabili subinhibitorno koncentracijo (0,1 MIK), saj nismo želeli vpliva ekstraktov na rast, temveč samo na adhezijo. Rezultati protimikrobnega delovanja so pokazali, da sta učinkovita ekstrakta CMAS in CMAC (CMA: ekstrakcija z mešanico kloroform, metanol, aceton; S: A. platensis; C: C. vulgaris) pri bakterijah vrste S. aureus (MIK = 10 mg/ml), medtem ko ostali ekstrakti niso imeli protimikrobnega učinka pri izbranih mikroorganizmih (MIK > 20 mg/ml). V nadaljevanju raziskave smo ugotovili, da protiadhezivno deluje ekstrakt MC (M: ekstrakcija z metanolom; C: C. vulgaris) pri bakterijah vrste S. aureus (28,8 % inhibicija), medtem ko je ekstrakt CMAS povečal adhezijo bakterij vrste S. aureus, in ekstrakt MS bakterijam vrst S. aureus in P. aeruginosa. Slednje bi lahko pripisali napaki pri meritvi absorpcije, do katere je prišlo zaradi temne obarvanosti obeh ekstraktov. Rezultati naše raziskave kažejo, da mikroalge vrst A. platensis in C. vulgaris predstavljajo velik potencial v razvoju novih in učinkovitih bioaktivnih sredstev, vendar so potrebne dodatne raziskave.

Language:Slovenian
Keywords:mikroalge, ekstrakti, bakterije, kvasovke, protimikrobno delovanje, protiadhezivno delovanje, biofilm
Work type:Master's thesis/paper (mb22)
Tipology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Year:2021
Publisher:[B. Grum]
Place:Ljubljana
UDC:579.24:582.2/.3
COBISS.SI-ID:78626563 This link opens in a new window
Views:56
Downloads:13
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Secondary language

Language:English
Title:Bioactive properties of Arthrospira platensis and Chlorella vulgaris extract
Abstract:
Microalgae are becoming increasingly popular in various scientific fields due to their ability to produce a wide range of organic compounds, among which many have bioactive properties. Among the mentioned compounds, those with antimicrobial and anti-adherence activities are particularly interesting. Such microalgae also include Arthrospira platensis and Chlorella vulgaris, whose bioactive properties against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans strains were researched in our study. We determined antimicrobial activity with minimum inhibitory concentration (MIC) of microalgae extracts with the broth microdilution method. We also determined antiadhesive activity with crystal violet staining method, for which we used sub-inhibitory concentration (0.1 MIC) of microalgae extracts, as we did not want to influence the growth but only adhesion of selected microorganisms. The results of antimicrobial activity showed that only CMAS and CMAC extracts (CMA: extraction with mixture of chloroform, methanol, acetone; S: A. platensis; C: C. vulgaris) were effective against S. aureus (MIC = 10 mg/ml), while other extracts had no antimicrobial effect against selected microorganisms (MIC > 20 mg/ml). In the continuation of our study, we found that MC extract had an antiadhesive effect against S. aureus (28.8% inhibition), while CMAS extract increased adhesion of S. aureus and MS extract increased adhesion of P. aeruginosa and S. aureus. This could be attributed to an error in absorbance measurement due to dark coloration of both extracts. Results of our research, together with results of researches of other authors proved that A. platensis and C. vulgaris microalgae represent a great potential in development of new and effective bioactive agents, but further researches are needed.

Keywords:microalgae, extracts, bacteria, yeasts, antimicrobial activity, antiadhesive activity, biofilm

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