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Tipizacija kriptosporidijev vrste Cryptosporidium parvum na podlagi analize večjega števila lokusov s spremenljivim številom tandemskih ponovitev
Golob, Klara (Author), Šoba Šparl, Barbara (Mentor) More about this mentor... This link opens in a new window

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Abstract
Praživali iz rodu Cryptosporidium povzročajo driske pri ljudeh in živalih po vsem svetu. Pri imunokompetentnih posameznikih povzročajo akutni, samoomejujoči gastroenteritis, pri imunsko oslabelih bolnikih pa je bolezen lahko kronična in življenjsko ogrožujoča. Okužba se prenaša z oocistami po fekalno-oralni poti. Kontaminirana voda je lahko pomemben vir velikih izbruhov bolezni. Za ugotavljanje virov kriptosporidioze in raziskave prenosa okužbe je multilokusna tipizacija najbolj informativno orodje. V naši raziskavi smo analizirali 73 izolatov DNA, pridobljenih iz blata 73 bolnikov, okuženih s Cryptosporidium parvum. Tipizacijo C. parvum na podlagi analize večjega števila lokusov s spremenljivim številom tandemskih ponovitev (MLVA) smo izvedli z določanjem dolžine fragmentov sedmih lokusov genoma parazita: cgd1, cgd4, cgd8, MM19, MSF, cgd5 in cgd6. Pri 39/73 (53 %) vzorcih smo uspešno določili dolžino fragmentov vseh sedmih lokusov. Pri teh 39 vzorcih smo ugotovili 25 različnih multilokusnih genotipov. Dvajset se jih je pojavilo enkrat, pet pa pri več kot enem bolniku. Med letoma 2010 in 2019 v Sloveniji ni bilo potrjenih izbruhov kriptosporidioze. Okužbe s kriptosporidiji v Sloveniji so večinoma sporadične. Kljub temu kaže, da so si lahko paraziti, izolirani iz blata bolnikov, ki živijo na istih ali bližnjih geografskih področjih, med seboj genetsko sorodni.

Language:Slovenian
Keywords:kriptosporidioza, Cryptosporidium parvum, driska, izbruhi, verižna reakcija s polimerazo, tipizacijske metode, kapilarna elektroforeza
Work type:Master's thesis/paper (mb22)
Tipology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Year:2021
Publisher:[K. Golob]
Place:Ljubljana
UDC:616.993.19-078:577.2.083
COBISS.SI-ID:78011395 This link opens in a new window
Views:82
Downloads:0
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Secondary language

Language:English
Title:Typing of Cryptosporidium parvum based on multiple-locus variable number tandem repeat analysis
Abstract:
Protozoa of the genus Cryptosporidium are associated with diarrhea in humans and animals worldwide. They are causative agents of acute, self-limiting gastroenteritis in immunocompetent individuals. In immunocompromised patients, the disease can be chronic and life-threatening. Infectious oocysts are transmitted through the faecal-oral route. Contaminated water sources are an important factor in large waterborne disease outbreaks. Multi-locus genotyping is the most informative tool for identifying sources of cryptosporidiosis and studying infection transmission. In this study, we analysed 73 DNA isolates from the faeces of 73 patients infected with Cryptosporidium parvum. Typing of C. parvum based on the multiple-locus variable number tandem repeat analysis (MLVA) was performed by determining the length of fragments of seven loci of the parasite genome: cgd1, cgd4, cgd8, MM19, MSF, cgd5 and cgd6. In 39/73 (53%) samples, the length of the fragments of all seven loci was successfully determined. In these 39 samples, 25 different multi-locus genotypes were identified. Twenty occurred only once and five appeared in more than one patient. Between 2010 and 2019, no outbreaks of cryptosporidiosis were confirmed in Slovenia. Cryptosporidium infections in Slovenia are mostly sporadic. Nevertheless, it appears that parasites isolated from the faeces of patients living in the same or a nearby geographical area may be genetically related.

Keywords:cryptosporidiosis, Cryptosporidium parvum, diarrhea, outbreaks, polymerase chain reaction, typing methods, capillary electrophoresis

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