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Možnosti pospešitve generacijskega cikla donorskih rastlin oljne ogrščice (Brassica napus L.) za hitrejše pridobivanje homozigotnih rastlin
ID Podgoršek, Klementina (Author), ID Murovec, Jana (Mentor) More about this mentor... This link opens in a new window

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Abstract
Žlahtnjenje novih sort, še posebej homozigotnih linij, je dolgotrajen proces, saj je število generacij rastlin na leto omejeno. S podaljševanjem fotoperiode in povečanjem gostote zasaditve smo želeli pospešit generacijski cikel donorskih rastlin oljne ogrščice (Brassica napus L.) ter preveriti ali to vpliva na indukcijo haploidov preko kulture mikrospor, ki je najbolj uporabljena metoda za pridobivanje homozigotnih rastlin oljne ogrščice. Rastline smo osvetljevali s 16 urno ali 22 urno fotoperiodo in sadili 1 ali 3 rastline na lonček (premera 13 cm). Ko sta se odprla prva 2 cvetova, smo nadaljevali z izolacijo in gojenjem mikrospor po protokolu, ki ga je objavil Custers (2003). Ugotovili smo, da so rastline pod 22 urno fotoperiodo zacvetele hitreje, gostota sajenja pa ni imela vpliva na hitrejšo indukcijo cvetenja. Ugotovili smo tudi, da smo pridobili največ embrijev rastlino pri rastlinah pod 22 urno fotoperiodo in pri višji gostoti sajenja. Čeprav smo uspešno pospešili generacijski čas donorskih rastlin, so bile te zakrnele z majhnim številom brstov, kar ni primerno za žlahniteljske tehnike, kjer so potrebne večje količine brstov in semen.

Language:Slovenian
Keywords:Brassica napus, oljna ogrščica, pospeševanje rasti, fotoperioda, haploidi, mikrospore
Work type:Master's thesis/paper (mb22)
Typology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Year:2021
Publisher:[K. Podgoršek]
Place:Ljubljana
UDC:635.44:631.528.69:575.113.2(043.2)
COBISS.SI-ID:78213635 This link opens in a new window
Publication date in RUL:28.09.2021
Views:190
Downloads:36
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Secondary language

Language:English
Title:Attempts to accelerate the generation cycle of donor oilseed rape plants (Brassica napus L.) for faster production of homozygous plants
Abstract:
Breeding new varieties, especially homozygous lines, is a time-consuming process, as the number of plant generations per year is limited. By prolonging the photoperiod and increasing the planting density, we aimed to accelerate the 18generation cycle of donor plants of oilseed rape (Brassica napus L.) and check whether this affects the induction of haploids through microspore culture, which is the most used method for obtaining homozygous oilseed rape plants. Plants were illuminated with 16-hour or 22-hour photoperiods and planted at a density of 1 or 3 plants per pot (diameter 13 cm). When 2 flowers appeared, we continued to isolate and grow microspores according to the protocol published by Custers (2003). We found that plants bloomed faster under the 22-hour photoperiod, and that planting density had no effect on faster induction of flowering. We also found that we obtained the highest number of embryos per bud from plants grown under the 22-hour photoperiod and at higher planting densities. Although we have successfully accelerated generation time of the donor plants, they were small with a low number of buds, which is not suitable for breeding techniques where larger quantities of buds and seeds are required.

Keywords:Brassica napus, oilseed rape, speed breeding, fotoperiod, haploids, microspores

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