Razvoj in uporaba elektrode z ogljikovo pasto in glukoza oksidazo za zaznavanje glukoze

Drinovec, Lara (author), Kolar, Mitja (mentor)

V voltametriji so se po Hg uveljavili novi elektrodni materiali na osnovi C, Bi, Sn itd. Predvsem Bi in modifikacije C kažejo vrsto prednosti in se jih uporablja za določanje biološko pomembnih analitov, kot so na primer kovinski ioni, nitrofenoli in glukoza. Med biosenzorji imajo največji pomen senzorji za zaznavanje glukoze, katerih ključni element je glukoza oksidaza. V sklopu diplomskega dela sem študirala lastnosti elektrodnih materialov na osnovi ogljika, različne tehnike snemanja (CV, SWV, DPV, AdV) in odziv na biološko pomemben analit. Pripravila sem umeritveno krivuljo za dodatke glukoze, izračunala stehiometrijo in določila LOD.

• Language: Slovenian
• Keywords: elektroda iz ogljikove paste, glukoza oksidaza, grafit, voltametrija, glukoza
• Work type: Bachelor thesis/paper
• Typology: 2.11 - Undergraduate Thesis
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Year: 2021
• PID: 20.500.12556/RUL-129976
• COBISS.SI-ID: 82917379
• Publication date in RUL: 09.09.2021

Secondary language

• Language: English
• Title: The development and use of carbon-based electrode materials for measurements in biochemistry

Abstract

New electrode materials based on C, Bi, Sn, etc. have been established in voltammetry, after Hg. In particular, Bi and C modifications show a number of advantages and are used to determine biologically important analytes such as metal ions, nitrophenols and glucose. Glucose sensors with glucose oxidase are the most important among biosensors. I have studied the properties of carbon-based electrode materials, various recording techniques (CV, SWV, DPV, AdV) and the response to a biologically important analyte. I prepared a calibration curve for glucose supplements, calculated stoichiometry, and determined LOD.

• Keywords: carbon paste electrode, glucose oxidase, graphite, voltammetry, glucose