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Macro- and microelements in serum and seminal plasma as biomarkers for bull sperm cryotolerance
ID Zakošek, Maja (Author), ID Zrimšek, Petra (Author), ID Jakovac-Strajn, Breda (Author), ID Pavšič Vrtač, Katarina (Author), ID Knific, Tanja (Author), ID Mrkun, Janko (Author)

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Abstract
ABSTRACT Background Wide variation in fertility rates is observed when using frozen bull semen, even when the bulls have met quality standards for semen production. Therefore, a simple and reliable test to assess the freezing potential of bull semen based on the analysis of fresh semen or blood would be of great value. Attention is now turning to assessment of seminal plasma components such as proteins and elements. In the present study, the concentrations of macro- and microelements in fresh bull semen plasma and in serum and their correlation with quality characteristics of fresh semen and with semen quality after freezing and thawing were determined. Ejaculates were collected from 30 mature bulls, and semen volume, concentration, sperm motility, morphology, tail membrane integrity, plasma membrane permeability and DNA fragmentation were determined on the day of collection and after freezing and thawing. The concentrations of macroelements (Na, Mg, K and Ca) and microelements (Cu, Fe, Zn and Se) were determined in the seminal plasma and serum. The semen samples were classified into satisfactory and unsatisfactory groups according to the fresh semen quality. Results Zinc and Se levels measured in serum were associated with almost all fresh and frozen-thawed semen quality characteristics, while Fe levels were associated only with acrosomal defects in fresh semen. Zinc and Fe levels in fresh seminal plasma were associated with various quality characteristics of fresh and frozen-thawed semen, while Se level in fresh seminal plasma was not associated with any of the semen quality characteristics. Conclusions Microelements were shown to be useful as biomarkers involved in the analysis of bull sperm quality and could be used as an additional tool to predict bull semen quality after freezing and thawing. Our results confirm that the analysis of Zn and Se levels in serum and Zn, Cu and Fe levels in fresh seminal plasma can provide information to discriminate between bull semen samples with spermatozoa with high or low cryotolerance.

Language:English
Keywords:Blood serum, bull semen, cryopreservation, microelements, quality, seminal plasma
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:VF - Veterinary Faculty
Publication status:Published
Publication version:Version of Record
Submitted for review:16.12.2020
Article acceptance date:25.06.2021
Publication date:05.07.2021
Year:2021
Number of pages:10 str.
Numbering:Vol. 63, art. 25
PID:20.500.12556/RUL-128770 This link opens in a new window
UDC:636.09:618.1
ISSN on article:1751-0147
DOI:10.1186/s13028-021-00590-2 This link opens in a new window
COBISS.SI-ID:69802499 This link opens in a new window
Publication date in RUL:28.07.2021
Views:722
Downloads:161
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Record is a part of a journal

Title:Acta veterinaria Scandinavica
Shortened title:Acta vet. Scand.
Publisher:BioMed Central
ISSN:1751-0147
COBISS.SI-ID:515671577 This link opens in a new window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:05.07.2021

Projects

Funder:ARRS - Slovenian Research Agency
Project number:P4-0053
Name:Endokrini, imunski in encimski odzivi pri zdravih in bolnih živalih

Funder:ARRS - Slovenian Research Agency
Project number:P4-0092
Name:Zdravje živali, okolje in varna hrana

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