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Identification of novel reassortant mammalian orthoreoviruses from bats in Slovenia
ID Mikuletič, Tina (Author), ID Černe, Danijela (Author), ID Hostnik, Peter (Author), ID Toplak, Nataša (Author), ID Koren, Simon (Author), ID Kuhar, Urška (Author), ID Jamnikar Ciglenečki, Urška (Author), ID Kutnjak, Denis (Author), ID Steyer, Andrej (Author)

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Abstract
Background: Recently, mammalian orthoreoviruses (MRVs) were detected for the first time in European bats, and the closely related strain SI-MRV01 was isolated from a child with severe diarrhoea in Slovenia. Genetically similar strains have also been reported from other mammals, which reveals their wide host distribution. The aim of this study was to retrospectively investigate the occurrence and genetic diversity of MRVs in bats in Slovenia, from samples obtained throughout the country in 2008 to 2010, and in 2012 and to investigate the occurrence of the novel SI-MRV01 MRV variant in Slovenian bats. Results: The detection of MRVs in bat guano was based on broad-range RT-PCR and specific bat MRV real-time RT-PCR. Subsequently, MRV isolates were obtained from cell culture propagation, with detailed molecular characterisation through whole-genome sequencing. Overall, bat MRVs were detected in 1.9% to 3.8% of bats in 2008, 2009 and 2012. However, in 2010 the prevalence was 33.0%, which defined an outbreak of the single SI-MRV01 strain. Here, we report on the identification of five MRV isolates of different serotypes that are designated as SI-MRV02, SI-MRV03, SI-MRV04, SI-MRV05 and SI-MRV06. There is high genetic variability between these characterised isolates, with evident genome reassortment seen across their genome segments. Conclusions: In conclusion, we have confirmed the presence of the SI-MRV01 strain in a Slovenian bat population. Moreover, according to genetic characterisation of S1 genome segment, all three MRV serotypes were present in the bat population. In this study, five independent MRV isolates were obtained and detailed whole genome analysis revealed high diversity between them. This study generates new information about the epidemiology and molecular characteristics of emerging bat MRV variants, and provides important molecular data for further studies of their pathogenesis and evolution.

Language:English
Keywords:bats, genome reassortment, mammalian orthoreovirus, whole genome sequencing
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:MF - Faculty of Medicine
VF - Veterinary Faculty
Publication status:Published
Publication version:Version of Record
Year:2018
Number of pages:10 str.
Numbering:Vol. 14, art. 264
PID:20.500.12556/RUL-128741 This link opens in a new window
UDC:575.111
ISSN on article:1746-6148
DOI:10.1186/s12917-018-1585-y This link opens in a new window
COBISS.SI-ID:33906649 This link opens in a new window
Publication date in RUL:27.07.2021
Views:955
Downloads:154
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Record is a part of a journal

Title:BMC veterinary research
Publisher:BioMed Central.
ISSN:1746-6148
COBISS.SI-ID:515674393 This link opens in a new window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:03.09.2018

Secondary language

Language:Slovenian
Keywords:netopirji, preureditev gena, ortoreovirus sesalcev, sekvenciranje celotnega genoma

Projects

Funder:ARRS - Slovenian Research Agency
Project number:P3-0083
Name:Odnosi parazitskega obstajanja

Funder:ARRS - Slovenian Research Agency
Project number:P4-0092
Name:Zdravje živali, okolje in varna hrana

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