izpis_h1_title_alt

Raznolikost genskih virov listnega ohrovta (Brassica oleracea L.)
ID Šušteršič, Jan (Author), ID Meglič, Vladimir (Mentor) More about this mentor... This link opens in a new window, ID Sinkovič, Lovro (Comentor)

.pdfPDF - Presentation file, Download (3,00 MB)
MD5: 0AC7F83B17F789CB8EE9B5838657C597

Abstract
Listni ohrovt (Brassica oleracea L.) je manj razširjena vrtnina, ki v zadnjem času vse bolj pridobiva na pomenu kot del zdrave prehrane, saj vsebuje številne zdravju koristne snovi. Namen magistrskega dela je bil preučiti raznolikost 18 genskih virov listnega ohrovta iz genskih bank v jugovzhodne Evrope. Na morfološkem nivoju smo raznolikost določali z uporabo 36 IBPGR deskriptorjev v vegetativni in generativni fazi rasti. Na biokemijskem nivoju smo raznolikost ovrednotili z meritvami vsebnosti skupnih fenolnih spojin s pomočjo Folin-Ciocalteu reagenta, antioksidacijskega potenciala po DPPH metodi in vsebnosti skupnih flavonoidov z aluminijevim kloridom. Stopnjo genetske raznolikosti smo določili z uporabo mikrosatelitnih markerjev. Pri vsakem obravnavanem genskemu viru smo v sklopu morfološke karakterizacije v vegetativni fazi rasti izmerili 9 numeričnih morfoloških parametrov, v generativni fazi pa ovrednotili 18 opisnih in izmerili 9 numeričnih morfoloških parametrov. Na podlagi rezultatov smo genske vire razvrstili in opisali glede na morfološke značilnosti. Največjo raznolikost na biokemijskem nivoju smo opazili pri antioksidacijskem potencialu in najmanjšo pri vsebnosti skupnih flavonoidov. Z mikrosateliti smo določili stopnjo raznolikosti med posameznimi genskimi viri listnega ohrovta in oblikovali tri genetske skupine. Pridobljene informacije in rezultati magistrskega dela bodo osnova za opis genskih virov listnatega ohrovta v različnih bazah podatkov ter uporabni pri nadaljnjih programih žlahtnjenja.

Language:Slovenian
Keywords:listni ohrovt, rastlinski genski viri, deskriptor, morfološka raznolikost, bioaktivne spojine, genetska raznolikost
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Place of publishing:Ljubljana
Publisher:[J. Šušteršič]
Year:2021
PID:20.500.12556/RUL-128534 This link opens in a new window
UDC:582.683.211.4:635.347:577.21:631.52:581.4:547.972(043.2)
COBISS.SI-ID:72146947 This link opens in a new window
Publication date in RUL:18.07.2021
Views:1332
Downloads:147
Metadata:XML RDF-CHPDL DC-XML DC-RDF
:
Copy citation
Share:Bookmark and Share

Secondary language

Language:English
Title:Diversity od genetic resources of leafy kale (Brassica oleracea L.)
Abstract:
Leafy kale (Brassica oleracea L.) is a less common vegetable that is recently gaining in importance as part of a healthy diet since it contains many health-promoting substances. The purpose of the master's thesis was to examine the diversity of 18 genetic resources of leafy kale from gene banks of South-Eastern Europe. At the morphological level, diversity was determined using 36 IBPGR descriptors during the vegetative and generative growth phases. At the biochemical level, diversity was evaluated by measuring the content of total phenolic compounds using Folin-Ciocalteu reagent, antioxidant potential according to the DPPH method and the content of total flavonoids using aluminium chloride. Genetic diversity was determined using microsatellite markers. Within the morphological characterisation, 9 numerical morphological parameters were measured for each genetic resource in the vegetative growth phase, and 18 descriptive and 9 numerical morphological parameters were described/measured in the generative phase. Based on the results, genetic resources were classified and described according to morphological characteristics. At the biochemical level, the highest diversity was observed for antioxidant potential and the lowest for the total flavonoid content. Genetic diversity between individual genetic resources of leafy kale was determined using microsatelites and three genetic groups were formed. The information obtained and the results of the master's thesis will be the basis for the description of genetic resources of leafy kales in databases and useful in further breeding programes.

Keywords:leafy kale, plant genetic resources, descriptor, morphological diversity, bioactive compounds, genetic diversity

Similar documents

Similar works from RUL:
Similar works from other Slovenian collections:

Back