Changes in the phenolic compounds of hop (Humulus lupulus L.) induced by infection with Verticillium nonalfalfae, the causal agent of hop Verticillium wilt

Kunej, Urban (author), Mikulič Petkovšek, Maja (author), Radišek, Sebastjan (author), Štajner, Nataša (author)

Phenolic compounds are involved in plant responses to various biotic and abiotic stress factors, with many studies suggesting their role in defense mechanisms against fungal pathogens. Soilborne vascular pathogen Verticillium nonalfalfae causes severe wilting and consequent dieback in a wide range of economically important crops, including hops (Humulus lupulus L.). In this study, we investigated the differential accumulation of phenolics in the susceptible “Celeia” and resistant “Wye Target” hop cultivars during the pathogenesis of Verticillium wilt. Quantitative polymerase chain reaction showed that colonization in the roots of both cultivars was intensive, but decreased continuously throughout the experiment in the resistant cultivar, while the relative fungal amount continuously increased in the stems of the susceptible cultivar. In response to colonization in the roots of the resistant cultivar, a significant increase in total flavanols was detected at three days postinoculation (dpi), suggesting a possible role in preventing fungus spread into the stems. The accumulation of phenolic compounds was less pronounced in the stems of the resistant cultivar since, compared to the latter, significant increases in flavonols at 3 and 15 dpi and hydroxycinnamic acids at 6 dpi were observed in the stems of the susceptible cultivar.

• Language: English
• Keywords: Humulus lupulus, Verticillium nonalfalfae, fungal infection, phenolic compounds, plant–pathogen interactions
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2020
• Number of pages: 16 str.
• Numbering: Vol. 9, iss. 7, art. 841
• PID: 20.500.12556/RUL-128262
• UDC: 577.2
• ISSN on article: 2223-7747
• DOI: 10.3390/plants9070841
• COBISS.SI-ID: 22285315
• Publication date in RUL: 07.07.2021

Record is a part of a journal

• Title: Plants
• Shortened title: Plants
• Publisher: MDPI
• ISSN: 2223-7747
• COBISS.SI-ID: 523345433

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 01.07.2020

Secondary language

• Language: Slovenian
• Keywords: Humulus lupulus, hmelj, Verticillium nonalfalfae, verticilijska uvelost, fenolne spojine, interakcija rastlina-patogen