Your browser does not allow JavaScript!
JavaScript is necessary for the proper functioning of this website. Please enable JavaScript or use a modern browser.
Open Science Slovenia
Open Science
DiKUL
slv
|
eng
Search
Browse
New in RUL
About RUL
In numbers
Help
Sign in
The Escherichia coli colibactin resistance protein ClbS is a novel DNA binding protein that protects DNA from nucleolytic degradation
ID
Molan, Katja
(
Author
),
ID
Podlesek, Zdravko
(
Author
),
ID
Hodnik, Vesna
(
Author
),
ID
Butala, Matej
(
Author
),
ID
Oswald, Eric
(
Author
),
ID
Žgur-Bertok, Darja
(
Author
)
PDF - Presentation file,
Download
(769,24 KB)
MD5: 57EC29A84460543A44B05975D5E4F225
URL - Source URL, Visit
https://www.sciencedirect.com/science/article/pii/S1568786418303021
Abstract
Cells employ specific and nonspecific mechanisms to protect their genome integrity against exogenous and endogenous factors. The clbS gene is part of the polyketide synthase machinery (pks genomic island) encoding colibactin, a genotoxin implicated in promoting colorectal cancer. The pks is found among the Enterobacteriaceae, in particular Escherichia coli strains of the B2 phylogenetic group. Several resistance mechanisms protect toxin producers against toxicity of their products. ClbS, a cyclopropane hydrolase, was shown to confer colibactin resistance by opening its electrophilic cyclopropane ring. Here we report that ClbS sustained viability and enabled growth also of E. coli expressing another genotoxin, the Usp nuclease. The recA::gfp reporter system showed that ClbS protects against Usp induced DNA damage. To elucidate the mechanism of ClbS mediated protection, we studied the DNA binding ability of the ClbS protein. We show that ClbS directly interacts with single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA), whereas ssDNA seems to be the preferred substrate. Thus, the ClbS DNA-binding characteristics may serve bacteria to protect their genomes against DNA degradation.
Language:
English
Keywords:
ClbS
,
DNA-binding protein
,
DNA damage protection
,
pks genomic island
,
Escherichia coli
Work type:
Article (dk_c)
Typology:
1.01 - Original Scientific Article
Organization:
BF - Biotechnical Faculty
Year:
2019
Publication status in journal:
Published
Article version:
Publisher's version of article
Number of pages:
Str. 50-54
Numbering:
Vol. 79
PID:
20.500.12556/RUL-128248
UDC:
579
ISSN on article:
1568-7856
DOI:
10.1016/j.dnarep.2019.05.003
COBISS.SI-ID:
5079119
Publication date in RUL:
07.07.2021
Views:
372
Downloads:
101
Metadata:
Cite this work
Plain text
BibTeX
EndNote XML
EndNote/Refer
RIS
ABNT
ACM Ref
AMA
APA
Chicago 17th Author-Date
Harvard
IEEE
ISO 690
MLA
Vancouver
:
Kopiraj citat
Share:
AddThis uses cookies that require your consent.
Edit consent...
Record is a part of a journal
Title:
DNA Repair
Publisher:
Elsevier
ISSN:
1568-7856
COBISS.SI-ID:
24102405
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:
01.07.2019
Projects
Funder:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Project number:
P1-0198
Name:
Molekularno-biološke raziskave mikroorganizmov
Funder:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Project number:
J1-8150
Name:
"DNA sampling II":": Metoda za prepoznavo na DNA neposredno ali posredno vezanih proteinov v bakteriji
Funder:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Funding programme:
PhD grant
Similar documents
Similar works from RUL:
Similar works from other Slovenian collections:
Back