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Vpliv malega proteina DdrR na lastnosti LexA proteinu podobnih molekul pri bakteriji Acinetobacter baumannii
Šeme, Nuša (Author), Butala, Matej (Mentor) More about this mentor... This link opens in a new window

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Abstract
Encimi, sintetizirani v odzivu SOS, omogočijo bakterijam popravilo poškodb DNA in s tem adaptacijo na stresne okoljske razmere. Sevi bakterije Acinetobacter baumannii, ki so odporni proti številnim antibiotikom, sintetizirajo mali protein DdrR, ki je specifičen le za rod Acinetobacter, in skupaj s proteinom UmuDAb v tej bakteriji uravnava prepis genov odziva SOS. V delu smo želeli preveriti ali se protein DdrR neposredno veže s proteinom UmuDAb. Poleg tega smo želeli preveriti ali protein DdrR interagira tudi z represorjema, ki nadzirata lizogeni cikel dveh temperantnih profagov v tej bakteriji. V študiji smo z uporabo površinske plazmonske resonance ugotovili, da se DdrR z visoko afiniteto veže na vse analizirane represorje in, najverjetneje na tak način koregulira prepis bakterijskih in bakteriofagnih genov.

Language:Slovenian
Keywords:bakterijski odziv SOS, Acinetobacter baumannii, regulacija genov, represor UmuDAb, mali protein DdrR, fagni represorji
Work type:Master's thesis/paper (mb22)
Tipology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Year:2021
Publisher:[N. Šeme]
Place:Ljubljana
UDC:579.25/.26:577.112:577.2.088
COBISS.SI-ID:69310467 This link opens in a new window
Views:205
Downloads:28
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Secondary language

Language:English
Title:The influence of small protein DdRr on characteristics of the LexA-like proteins at Acinetobacter baumanii
Abstract:
Enzymes, synthesized in bacterial SOS response, enable bacterial organisms to repair DNA damage and therefore easily adapt to stresful enviroments and chemical agenses. Acinetobacter baumannii strains are resistant to a large range and variety of antibiotics. During SOS response Acinetobacter baumannii synthesize small protein molecule, named DdrR, which is specific only for genus Acinetobacter and can be found no where else. When synthesized, DdrR co-regulates transcription of SOS damage inducible genes together with main bacterial protein regulator, UmuDAb. In the study we examined if DdrR binds directly to UmuDAb protein and to two other phage repressors, which regulate lizogenic cycle of two temperate prophages, situated in bacterial chromosomal DNA. Surface plasmon resonance analysis indicated that DdrR protein binded succesfully to all analyzed protein repressors, with high affinity, and, in this way, it most likely co-regulates transcription of bacterial and bacteriophage genes.

Keywords:bacterial SOS response, Acinetobacter baumannii, regulation of genes, UmuDAb repressor, DdrR small protein, prophage repressors

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