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The first detection and genetic characterization of four different honeybee viruses in wild bumblebees from Croatia
ID Tlak Gajger, Ivana (Author), ID Šimenc Kramar, Laura (Author), ID Toplak, Ivan (Author)

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Abstract
To determine the presence and the prevalence of four different honeybee viruses (acute bee paralysis virus—ABPV, black queen cell virus—BQCV, chronic bee paralysis virus—CBPV, deformed wing virus—DWV) in wild bumblebees, pooled randomly selected bumblebee samples were collected from twenty-seven different locations in the territory of Croatia. All samples were prepared and examined using the RT-PCR methods for quantification of mentioned honeybee viruses. Determined prevalence (%) of identified positive viruses were in the following decreasing order: BQCV > DWV > ABPV, CBPV. Additionally, direct sequencing of samples positive for BQCV (n = 24) and DWV (n = 2) was performed, as well as a test of molecular phylogeny comparison with those available in GenBank. Selected positive field viruses’ strains showed 95.7 to 100% (BQCV) and 98.09% (DWV) nucleotide identity with previously detected and deposited honeybee virus strains in the geographic areas in Croatia and neighboring Slovenia. In this article, the first detection of four honeybee viruses with genetic characterization of high diversity strains circulating in wild bumblebees in Croatia is presented.

Language:English
Keywords:Bombus spp., honeybee viruses, deformed wing virus, black queen cell virus, acute bee paralysis virus, chronic bee paralysis virus, genetic characterization, sequencing, transmission routes, viruses, genetics, veterinary epidemiology, bees, virology, bumblebees
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:VF - Veterinary Faculty
Publication status:Published
Publication version:Version of Record
Year:2021
Number of pages:11 str.
Numbering:Vol. 10, iss. 7, art. 808
PID:20.500.12556/RUL-128032 This link opens in a new window
UDC:638:578
ISSN on article:2076-0817
DOI:10.3390/pathogens10070808 This link opens in a new window
COBISS.SI-ID:68557315 This link opens in a new window
Publication date in RUL:01.07.2021
Views:1750
Downloads:215
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Record is a part of a journal

Title:Pathogens
Shortened title:Pathogens
Publisher:MDPI
ISSN:2076-0817
COBISS.SI-ID:523338009 This link opens in a new window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:01.07.2021

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