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Genomic insights into the emergence and spread of methicillin-resistant Staphylococcus pseudintermedius in veterinary clinics
ID
Papić, Bojan
(
Author
),
ID
Golob, Majda
(
Author
),
ID
Zdovc, Irena
(
Author
),
ID
Kušar, Darja
(
Author
),
ID
Avberšek, Jana
(
Author
)
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https://www.sciencedirect.com/science/article/pii/S0378113521001425
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Abstract
Staphylococcus pseudintermedius is a common cause of skin and soft tissue infections in dogs but can also cause infections in cats and humans. The frequency of methicillin-resistant S. pseudintermedius (MRSP) strains is increasing worldwide. Here, we obtained 43 MRSP isolates from dogs (n = 41), one cat (n = 1) and the small animal clinic environment (n = 1) in Slovenia from the period 2008–2018, which underwent whole-genome sequencing (WGS) and antimicrobial susceptibility testing. Five sequence types (STs) were identified, with ST71 (32/43) and ST551 (8/43) being the predominant. In Slovenia, ST551 was first detected in 2016, whereas a decrease in the frequency of ST71 was observed after 2015. All isolates were multidrug-resistant and most antimicrobial-resistant phenotypes could be linked to acquisition of the corresponding resistance genes or gene mutations. Core-genome multilocus sequence typing (cgMLST) revealed several potential MRSP transmission routes: (i) between two veterinary clinics by a single MRSP-positive dog, (ii) between the environment of a veterinary clinic and a dog, and (iii) between a canine and a feline patient through the contaminated environment of a veterinary clinic. Of the six dogs that were additionally sampled from 14 days to five months after the initial sampling, each harbored the same MRSP strain, suggesting a limited within-host diversity of MRSP in symptomatic dogs. The present results highlight the importance of MRSP-positive dogs in the spread of veterinary care-associated MRSP infections and call for the implementation of strict control measures to reduce MRSP contamination in veterinary clinic environments originating from animal-contact surfaces.
Language:
English
Keywords:
antimicrobial susceptibility testing
,
epidemiology
,
methicillin-resistant Staphylococcus pseudintermedius (MRSP)
,
small animal clinic
,
whole-genome sequencing (WGS)
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
VF - Veterinary Faculty
Publication status:
Published
Publication version:
Version of Record
Year:
2021
Number of pages:
7 str.
Numbering:
Vol. 258, art. 109119
PID:
20.500.12556/RUL-127443
UDC:
636.09:579
ISSN on article:
1873-2542
DOI:
10.1016/j.vetmic.2021.109119
COBISS.SI-ID:
64516867
Publication date in RUL:
07.06.2021
Views:
1495
Downloads:
251
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Record is a part of a journal
Title:
Veterinary microbiology
Publisher:
Elsevier
ISSN:
1873-2542
COBISS.SI-ID:
23130885
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Projects
Funder:
ARRS - Slovenian Research Agency
Project number:
J4-8224
Name:
Ugotavljanje in karakterizacija povzročiteljev kužnih bolezni z metodo naslednje generacije sekvenciranja
Funder:
ARRS - Slovenian Research Agency
Project number:
P4-0092
Name:
Zdravje živali, okolje in varna hrana
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