Ugotavljanje vsebnosti rutina, kvercetina in kvercitrina v izvlečkih navadne ajde, (Fagopyrum esculentum) pridobljenih z ekstrakcijo s superkritičnim ogljikovim dioksidom

Bolčina, Manca (author), Kočevar Glavač, Nina (mentor)

Ajda (Fagopyrum sp.) je rastlina, ki jo že vrsto let gojimo za uporabo v prehrani in farmaciji. V zadnjem času njeni koristni učinki na zdravje ljudi prihajajo še bolj v ospredje. Poleg visokega deleža proteinov in mineralov je znana po veliki vsebnosti fenolnih spojin, predvsem rutina, ki ga največ najdemo v njeni zeli. Njena raznolika sestava bioaktivnih snovi pozitivno vpliva na potek pogostih bolezenskih stanj, kot so povišan krvni tlak, hiperholesterolemija in sladkorna bolezen. V raziskavah so dokazali tudi nevroprotektivno, protitumorno in protivnetno delovanje. V magistrski nalogi smo ugotavljali vsebnosti flavonoidov rutina, kvercetina in kvercitrina ter fagopirinov v ekstraktih navadne ajde (Fagopyrum esculentum) sorte Darja, pridobljenih z ekstrakcijo s superkritičnim CO2 in etanolom. Vsebnost proučevanih spojin smo ugotavljali v ekstraktih listov, cvetov, nezrelih in zrelih plodov ter ajdovi kaši z analizo konvergenčne kromatografije ultravisoke ločljivosti (UPC2) in tekočinske kromatografije visoke ločjlivosti (HPLC). Za podrobnejši vpogled v sestavo vzorcev smo izvedli tudi analizo z GC-MS. Flavonoidov nismo detektirali pri vseh vzorcih. Največjo vsebnost (52,54 mg/g droge) smo ugotovili pri suhih listih navadne ajde. Od vseh treh flavonoidov se je najpogosteje pojavljal kvercitrin, in sicer največ v etanolnem ekstraktu cvetov frakcije CV1 (2,63 mg/g droge). Izmed CO2 in etanolnih ekstraktov je imel največ rutina etanolni ekstrakt listov frakcije CV1 (0,23 mg/g droge), največ kvercetina pa etanolni ekstrakt listov frakcije CV3 (0,50 mg/g droge). Povzamemo lahko, da so pri navadni ajdi (F. esculentum) sorte Darja s flavonoidi najbolj bogati listi in cvetovi, najrevnejša pa kaša. V nasprotju s flavonoidi smo fagopirine detektirali pri vseh vzorcih. Največjo vsebnost smo ugotovili v CO2 ekstraktu listov frakcije CV1 (25,54 mg/g droge). Največ spojin (21) smo identificirali pri etanolnem ekstraktu listov frakcije CV3, najmanj pa pri kaši. Največ neidentificiranih snovi (18) smo analizirali pri etanolnem ekstraktu cvetov frakcije CV1. Delež identificiranih snovi je bil skoraj pri vseh analiziranih ekstraktih, razen pri kaši, okoli 80 %.

• Language: Slovenian
• Keywords: Ajda, flavonoidi, superkritična ekstrakcija, rutin, kvercetin, kvercitrin
• Work type: Master's thesis/paper
• Organization: FFA - Faculty of Pharmacy
• Year: 2021
• PID: 20.500.12556/RUL-127217
• Publication date in RUL: 26.05.2021

Secondary language

• Language: English
• Title: Determination of rutin, quercetin and quercitrin in common buckwheat (Fagopyrum esculentum) extracts obtained with supercritical carbon dioxide extraction

Abstract

Buckwheat (Fagopyrum sp.) is a plant that has been grown for its use in food and pharmacy for years. Lately, it has made it to the forefront with its beneficial effects on human health. Additionally to its high levels of proteins and minerals it is known for its high content of phenolic compounds, especially rutin, which is mostly found in its herb. Its diverse composition of bioactive substances has a positive effect on the development of common disease states like high blood pressure, hypercholesterolemia and diabetes. It is also attributed with neuroprotective, antitumor and antiinflammatory effects. In this master's thesis we determined the flavonoid contents of rutin, quercetin and quercitrin along with fagopyrins found in the common buckwheat (Fagopyrum esculentum) extracts of the Darja variety, that were obtained by extraction with supercritical CO2 and ethanol. The content of the studied compounds was determined in the extracts of leaves, flowers, unripened and ripened fruits as well as in buckwheat pulp with UPC2 and HPLC analyses. We also performed a GC-MS analysis for a more detailed insight into the composition of the samples. The flavonoids were not detected in all samples. The highest content (52,54 mg/g of the drug) was determined in dry leaves of common buckwheat. Out of all three flavonoids, we most frequently measured the content of quercitrin namely in the ethanolic flower extract of the CV1 fraction (2,63 mg/g of the drug). Out of the CO2 and ethanol extracts, the most rutin is in the ethanolic leaves extract of the CV1 fraction (0,23 mg/g of the drug), and the most of the quercetin is in the ethanolic leaves extract of the fraction CV3 (0,50 mg/g of the drug). We can summarize that in the common buckwheat (F. esculentum) of the Darja variety, leaves and flowers come in with the highest flavonoid levels while the pulp comes in last with the lowest amounts. In contrast to flavonoids, fagopyrins were detected in all samples. The highest content was found in the CO2 leaves extract of the CV1 fraction (25,54 mg/g of the drug). Most compounds (21) were identified in the ethanolic leaves extract of the CV3 fraction, and at the least here in the pulp. Most unidentified substances (18) were analyzed in the ethanolic flower extract of the CV1 fraction. The proportion of identified substances in almost all analyzed extracts, except for pulp, is about 80 %.

• Keywords: Buckwheat, flavonoids, supercritical extraction, rutin, quercetin, quercitrin