A rapid analysis of bisphenol A using MISPE coupled with HPLC-FLD in tissues of food-producing animals

Cerkvenik, Vesna (author), Šturm, Sabina (author)

Bisphenol A (BPA) is a highly-produced organic compound of anthropogenic origin with a wide-range use and is ubiquitously present in both living organisms and the environment. A previously published analytical method for testing of the free (aglycone) BPA in foodstuffs was simplified and optimized for sheep muscle tissue, kidney and liver, by using only a single MISPE purification step allied with HPLC-FLD analysis. The recovery rates and RSD values over a concentration range of 1–10 μg/kg were in the range of 67–86% and 3–34%, respectively, while linearity in the matrix, represented by the r$^2$, was ≥0.999. LOD values were 0.5–1 μg/kg and LOQ values were 1 μg/kg. The analytical method used is a contribution to the field of veterinary toxicology and food testing and also proved to be applicable for other food-producing animal species, e.g. pigs, poultry and freshwater fish. The MISPE sorbent material for testing of BPA was reusable, with up to five re-use cycles without significant loss in performance characteristics. •The paper reports on a rapid determination of free (aglycone) BPA in tissues of food-producing animals by HPLC-FLD and presents a substantial improvement of analytical performance with regard to time- and cost-savings, as well as environmental protection. •MISPE is an advanced analytical technology, and the results proved that in a single and simplified SPE step it had good performance characteristics such as selectivity, recovery and precision, allied with low LOD and LOQ values. •It was proved that MISPE BPA cartridges could be used at least five times without significant loss of methodological recovery and precision.

• Language: English
• Keywords: bisphenol A, tissue analysis, solid phase extraction, molecularly imprinted polymers, liquid chromatography, bisphenol A-glycidyl methacrylate
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: 14 str.
• Numbering: Vol. 8, art. 101351
• PID: 20.500.12556/RUL-127091
• UDC: 615.9
• ISSN on article: 2215-0161
• DOI: 10.1016/j.mex.2021.101351
• COBISS.SI-ID: 62349827
• Publication date in RUL: 17.05.2021

Record is a part of a journal

• Title: MethodsX
• Publisher: Elsevier
• ISSN: 2215-0161
• COBISS.SI-ID: 13645083

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0092
• Name: Zdravje živali, okolje in varna hrana