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Bioprospecting of less-polar constituents from endemic brown macroalga Fucus virsoides J. Agardh from the Adriatic Sea and targeted antioxidant effects in vitro and in vivo (zebrafish model)
ID
Jerković, Igor
(
Author
),
ID
Cikoš, Ana-Marija
(
Author
),
ID
Babić, Sanja
(
Author
),
ID
Čižmek, Lara
(
Author
),
ID
Bojanić, Krunoslav
(
Author
),
ID
Aladić, Krunoslav
(
Author
),
ID
Ul'yanovskii, Nikolai
(
Author
),
ID
Kosyakov, Dmitry
(
Author
),
ID
Lebedev, Albert T.
(
Author
),
ID
Čož-Rakovac, Rozelindra
(
Author
),
ID
Trebše, Polonca
(
Author
),
ID
Jokić, Stela
(
Author
)
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https://www.mdpi.com/1660-3397/19/5/235
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Abstract
The endemic brown macroalga Fucus virsoides J. Agardh from the Adriatic Sea was in the focus of the present research. The volatiles of fresh (FrFv) and air-dried (DrFv) samples of F. virsoides obtained by headspace solid-phase microextraction (HS-SPME) and hydrodistillation (HD) were analyzed by gas chromatography equipped with flame ionization detector and mass spectrometry (GC-FID/MS). The major HS-FrFv compound was pentadecane (61.90–71.55%) followed by pentadec1-ene (11.00–7.98%). In HS-DrFv, pentadec-1-ene was not present, and few lower aliphatic compounds appeared, as well as benzaldehyde and benzyl alcohol. In HD-FrFv, particularly abundant were alkenes (such as pentadec-1-ene (19.32%), or (E)-pentadec-7-ene (8.35%)). In HD-DrFv, more oxidation products were present (e.g., carbonyl compounds such as tridecanal (18.51%)). The fatty acids profile of freeze-dried sample (FdFv) after conversion to methyl esters was determined by GC-FID, and oleic acid was dominant (42.28%), followed by arachidonic acid (15.00%). High-performance liquid chromatography-high-resolution mass spectrometry with electrospray ionization (HPLC-ESI-HRMS) was used for the screening of less polar fractions (F3 and F4) of F. virsoides. Mono- and diglycerides of stearic, palmitic, oleic, and arachidonic acids were found. Terpenoids and steroids comprised the compounds C$_{20}$H$_{30(32)}$O$_2$ and C$_{29}$H$_{48}$O$_{(2)}$. Among carotenoids, fucoxanthin was identified. Chlorophyll derivatives were also found (C$_{55}$H$_{74(72)}$N$_4$O$_{(5-7)}$), dominated by pheophytin a. The antioxidant activity of the fractions was investigated by in vitro assays (oxygen radical absorbance capacity (ORAC), reduction of radical cation (ABTS•$^+$), 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay, and ferric reducing antioxidant power (FRAP)) and by in vivo zebrafish model (along with fish embryotoxicity). In vitro experiments proved good radical scavenging abilities of F3 and F4 fractions, which were additionally supported by the protective effect against hydrogen peroxideinduced oxidative stress in zebrafish embryos.
Language:
English
Keywords:
brown algae
,
volatiles
,
fatty acids
,
pigments
,
bioassays
,
embryotoxicity
,
reactive oxygen species
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
ZF - Faculty of Health Sciences
Publication status:
Published
Publication version:
Version of Record
Year:
2021
Number of pages:
25 str.
Numbering:
Vol. 19, iss. 5, art. 235
PID:
20.500.12556/RUL-126488
UDC:
543.51:582.272
ISSN on article:
1660-3397
DOI:
10.3390/md19050235
COBISS.SI-ID:
60999427
Publication date in RUL:
23.04.2021
Views:
1860
Downloads:
214
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Record is a part of a journal
Title:
Marine drugs
Shortened title:
Mar. drugs
Publisher:
MDPI
ISSN:
1660-3397
COBISS.SI-ID:
23578841
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:
01.05.2021
Projects
Funder:
Other - Other funder or multiple funders
Funding programme:
Croatian Government
Name:
The Scientific Centre of Excellence for Marine Bioprospecting
Acronym:
BioProCro
Funder:
EC - European Commission
Funding programme:
European Regional Development Fund, Competitiveness and Cohesion Operational Programme
Name:
The Scientific Centre of Excellence for Marine Bioprospecting
Acronym:
BioProCro
Funder:
Other - Other funder or multiple funders
Funding programme:
Russian Federation, Ministry of Science and Higher Education
Project number:
0793-2020-0007
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