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The role of buffers in wild-type HEWL amyloid fibril formation mechanism
ID Brudar, Sandi (Author), ID Hribar-Lee, Barbara (Author)

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Abstract
Amyloid fibrils, highly ordered protein aggregates, play an important role in the onset of several neurological disorders. Many studies have assessed amyloid fibril formation under specific solution conditions, but they all lack an important phenomena in biological solutions—buffer specific effects. We have focused on the formation of hen egg-white lysozyme (HEWL) fibrils in aqueous solutions of different buffers in both acidic and basic pH range. By means of UV-Vis spectroscopy, fluorescence measurements and CD spectroscopy, we have managed to show that fibrillization of HEWL is affected by buffer identity (glycine, TRIS, phosphate, KCl-HCl, cacodylate, HEPES, acetate), solution pH, sample incubation (agitated vs. static) and added excipients (NaCl and PEG). HEWL only forms amyloid fibrils at pH = 2.0 under agitated conditions in glycine and KCl-HCl buffers of high enough ionic strength. Phosphate buffer on the other hand stabilizes the HEWL molecules. Similar stabilization effect was achieved by addition of PEG12000 molecules to the solution.

Language:English
Keywords:lysozyme, amyloid fibrils, buffer-specific effects
Work type:Article (dk_c)
Typology:1.01 - Original Scientific Article
Organization:FKKT - Faculty of Chemistry and Chemical Technology
Year:2019
Publication status in journal:Published
Article version:Publisher's version of article
Number of pages:18 str.
Numbering:Vol. 9, iss. 2, art. 65
PID:20.500.12556/RUL-125374 This link opens in a new window
UDC:577.322
ISSN on article:2218-273X
DOI:10.3390/biom9020065 This link opens in a new window
COBISS.SI-ID:1538128835 This link opens in a new window
Publication date in RUL:12.03.2021
Views:455
Downloads:136
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Record is a part of a journal

Title:Biomolecules
Shortened title:Biomolecules
Publisher:MDPI AG
ISSN:2218-273X
COBISS.SI-ID:519952921 This link opens in a new window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:12.03.2021

Secondary language

Language:Slovenian
Keywords:lizocim, amiloidne fibrile, pufer-specifični efekti

Projects

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Project number:P1-0201
Name:Fizikalna kemija

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Project number:BI-US/18-19-061

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