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Vpliv sogojenja glive Monascus purpureus z izbranimi saprofitskimi glivami na biosintezo citrinina in monakolina K
ID Marič, Ajda (Author), ID Gregori, Andrej (Mentor) More about this mentor... This link opens in a new window, ID Sepčić, Kristina (Comentor)

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Abstract
Glavni namen naših raziskav je bil razviti postopek gojenja glive Monascus purpureus, s ciljem doseči čim manjšo vsebnost nefrotoksičnega metabolita citrinina, ne da bi se med tem delež farmakološko pomembnega statina monakolina K zmanjšal, oz. bi se morda povečal. Osredotočili smo se na vpliv sogojenja M. purpureus z različnimi vrstami zdravilnih saprofitskih gliv. Bazične študije so nam omogočile globlji vpogled v medsebojne vplive med glivami različnih vrst, s poudarkom na biosintezi citrinina in monakolina K. Optimizirano gojenje in sogojenje je potekalo v steklenih kozarcih, na mešanici prosa in riža pri različni začetni vsebnosti substrata. Ugotovili smo, da ima temperatura, čas inkubacijske dobe, sestava substrata, začetna vsebnost vode substrata, izbrani sev M. purpureus in gob Flammulina velutipes ter dvema sevoma Trametes versicolor, s katerimi je potekalo sogojenje, odvzem in priprava vzorcev za analizo, vpliv na biosintezo sekundarnih metabolitov (monakolina K, citrinina in pigmentov), ergosterola, vrednost pH, izgubo suhe mase in vsebnost vode v sveže preraščenem substratu.

Language:Slovenian
Keywords:Monascus poprureus, sogojenje, citrinin, monakolin K, celokupna barvila, proso, riž, optimizacija gojenja na trdni podlagi
Work type:Doctoral dissertation
Organization:BF - Biotechnical Faculty
Year:2020
PID:20.500.12556/RUL-124426 This link opens in a new window
Publication date in RUL:21.01.2021
Views:1479
Downloads:94
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Secondary language

Language:English
Title:Influence of co-cultivation of Monascus purpureus with selected saprophytic fungi on biosynthesis of citrinin and monacolin K
Abstract:
The main goal of our research was to develop a cultivation process of Monascus purpureus in which the chief premise was to achieve the lowest levels of nephrotoxic metabolite citrinin, while also making sure that the amount of pharmacologically significant important statin monacolin K did not decrease or, potentially, increase. Our primary focus was the effect of cocultivation of M. purpureus with different types of healing saprophytic fungi. Baseline studies made it possible for us to gain a deeper insight into the reciprocal effects between different types of fungi, more specifically focusing on the biosynthesis of citrinin and monacolin K. Optimised cultivation and cocultivation took place in glass jars and was carried out on a mixture of proso millet and rice at different initial water content in the substrate. Our findings show that temperature, the duration of the incubation time, substrate composition, initial water content in the substrate, the chosen strain of M. purpureus and the fungi Flammulina velutipes and Trametes versicolor with which M. purpureus was cocultivated and the process of handling samples for analysis are all factors that affect the biosynthesis of secondary metabolites (monacolin K, citrinin and pigments), ergosterol, pH value, the loss of dry mass and content of water in a freshly overgrown substrate.

Keywords:Monascus purpureus, cocultivation, citrinin, monacolin K, total pigments, millet, rice, optimization of a solid state fermentation

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