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Comparison of canine and feline adipose-derived mesenchymal stem cells/medicinal signaling cells with regard to cell surface marker expression, viability, proliferation, and differentiation potential
ID
Voga, Metka
(
Author
),
ID
Kovač, Valerija
(
Author
),
ID
Majdič, Gregor
(
Author
)
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https://www.frontiersin.org/articles/10.3389/fvets.2020.610240/full
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Abstract
Remarkable immunomodulatory abilities of mesenchymal stem cells, also called multipotent mesenchymal stromal cells or medicinal signaling cells (MSCs), have entailed significant advances in veterinary regenerative medicine in recent years. Despite positive outcomes from MSC therapies in various diseases in dogs and cats, differences in MSC characteristics between small animal veterinary patients are not well-known. We performed a comparative study of cells' surface marker expression, viability, proliferation, and differentiation capacity of adipose-derived MSCs (ADMSCs) from dogs and domestic cats. The same growth media and methods were used to isolate, characterize, and culture canine and feline ADMSCs. Adipose tissue was collected from 11 dogs and 8 cats of both sexes. The expression of surface markers CD44, CD90, and CD34 was detected by flow cytometry. Viability at passage 3 was measured with the hemocytometer and compared to the viability measured by flow cytometry after 1 day of handling. The proliferation potential of MSCs was measured by calculating cell doubling and cell doubling time from second to eighth passage. Differentiation potential was determined at early and late passages by inducing cells toward adipogenic, osteogenic, and chondrogenic differentiation using commercial media. Our study shows that the percentage of CD44+CD90+ and CD34−/− cells is higher in cells from dogs than in cells from cats. The viability of cells measured by two different methods at passage 3 differed between the species, and finally, canine ADMSCs possess greater proliferation and differentiation potential in comparison to the feline ADMSCs.
Language:
English
Keywords:
mesenchymal stem cells
,
dogs
,
cats
,
comparative study
,
stem cells
,
proliferation
,
differentiation
,
cell surface marker
,
viability
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
VF - Veterinary Faculty
Publication status:
Published
Publication version:
Version of Record
Year:
2021
Number of pages:
13 str.
Numbering:
Vol. 7, art. 610240
PID:
20.500.12556/RUL-124375
UDC:
636.09:575
ISSN on article:
2297-1769
DOI:
10.3389/fvets.2020.610240
COBISS.SI-ID:
46744323
Publication date in RUL:
19.01.2021
Views:
956
Downloads:
200
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Record is a part of a journal
Title:
Frontiers in veterinary science
Shortened title:
Front. vet. sci.
Publisher:
Frontiers Media S.A.
ISSN:
2297-1769
COBISS.SI-ID:
3969402
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:
19.01.2021
Projects
Funder:
ARRS - Slovenian Research Agency
Project number:
P4-0053
Name:
Endokrini, imunski in encimski odzivi pri zdravih in bolnih živalih
Funder:
ARRS - Slovenian Research Agency
Funding programme:
Mladi raziskovalci (Ph.D. fellowship)
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