Odziv entomopatogenih ogorčic (Steinernematidae in Heterorhabditidae) na izbrane kemične snovi, vezane na zeolit

Kunšek, Blažka (author), Laznik, Žiga (mentor)

Sproščanje hlapnih spojin (HS) iz napadenega ali nenapadenega tkiva rastlin vpliva tudi na entomopatogene ogorčice (EO). HS lahko na EO delujejo privabilno ali odvračalno. V našem poskusu smo preučevali tri vrste EO; S. feltiae, S. carpocapsae in H. bacteriophora in dve različni HS; terpinolen (T) in beta kariofilen (β-K). T izločajo nepoškodovane korenine korenja (Daucus carota L.) medtem ko β-K izločajo poškodovane korenine koruze (Zea mays L.), po napadu koruznega hrošča (Diabrotica virgifera virgifera, LeConte, 1868). HS smo vezali na različne granulacije zeolita. V raziskavi smo želeli ugotoviti ali HS T in β-K, vezani na različne granulacije zeolita, vplivata na gibanje EO podobno kot v vodnem mediju. Rezultati so pokazali, da je kemotaksični indeks (KI) odvisen od vrste EO, strategije iskanja gostitelja ter vrste HS. T v obeh preučevanih medijih (v vodi in vezan na zeolit) deluje kot repelent na EO H. bacteriophora in S. feltiae. β-K pa v obeh preučevanih medijih (v vodi in vezan na zeolit) deluje kot atraktant na EO H. bacteriophora in S. feltiae. Najmanj mobilna vrsta EO je bila S. carpocapsae. Ugotovili smo, da granulacija inertnega substrata (zeolit) nima vpliva na KI.

• Language: Slovenian
• Keywords: biotično varstvo, entomopatogene ogorčice, hlapne spojine, Steinernematidae, Heterorhabditidae, zeolit, terpinolen, beta kariofilen
• Work type: Master's thesis/paper
• Typology: 2.09 - Master's Thesis
• Organization: BF - Biotechnical Faculty
• Publisher: [B. Kunšek]
• Year: 2020
• PID: 20.500.12556/RUL-122214
• UDC: 632.937(043.2)
• COBISS.SI-ID: 41274883
• Publication date in RUL: 28.11.2020

Secondary language

• Language: English
• Title: Response of entomopathogenic nematodes (Steinernematidae in Heterorhabditidae) on selected chemical compounds, related to zeolite

Abstract

Releasing of volatile compounds (VC) from attacked or not attacked plant tissue influence on entomopathogenic nematodes (EN). VC may have an attractive or repulsive effect on EN. Three species of EN were investigated; S. feltiae, S. carpocapsae and H. bacteriophora and two different VC; terpinolene (T) and β-caryophyllene (β-K) in this trial. T is excreted by undamaged carrot (Daucus carota L.) roots while β-K is excreted by damaged maize (Zea mays L.) roots after beetle Diabrotica virgifera virgifera (LeConte, 1868) attack. VC were bound to different granulations of zeolite. During the trial it was tried to find out if VC, T and (β-K) bind to different granulations of zeolites influence EN movement similar as in water media. Results showed that chemotaxic indeks (KI) is dependent on species of EN, host searching strategy and sort of VC. T works in both investigated medias (water and zeolites) as a repellent on EN H. bacteriophora and S. feltiae. β-K works in both investigated medias (water and zeolites) as an atractant on EN H. bacteriophora and S. feltiae. The least mobile species EN was S. carpocapsae. It was found out that granulation of inert substrate (zeolites) has no effect on KI.

• Keywords: biological control, entomopathogenic nematodes, volatile compounds, Steinernematidae, Heterorhabditidae, zeolite, terpinolene, β-caryophyllene