Various environmental factors affect the health and production results of broilers, but one of the main factors is heat stress, which triggers oxidative processes in the animal`s body. Antioxidants are the main inhibitors of oxidation in the body. The main goal of the master`s thesis was to study the supplements of vitamins E, C Se and their combination in different concentrations on animal health and histological structure of the gastrointestinal tract. We were interested if under heat stress conditions, the NRC recommendations were sufficient to cover the antioxidant needs compared to the recommendations for Ross 308 chickens. The experiment included 120-day-old broilers, which were divided into 6 experimental groups (NRC, Ross, VitE, VitE, Se, and ECSe). During the experiment, we monitored mortality rate, feed consumption and conversion ratio, and weight gain. At the end of the experiment, we analysed the samples from blood serum, intestine, cecum and colon and their contents. Liver enzyme activity, intestinal viscosity, pH, SCFA concentration, and intestinal morphometric changes were measured. The results showed that feed supplementation with vitamin E, C, Se and their combination had no effect on histological changes of the gastrointestinal tract. There was a sufficient concentration of the studied vitamins and minerals in the recommended feed mixes of NRC and Ross, which covered the needs of broilers even under heat stress conditions.
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