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Preizkušanje virulentnosti izbranih izolatov entomopatogenih gliv iz rodov Beauveria in Metarhizium na ličinkah mokarja (Tenebrio molitor L.) in voščene vešče (Galleria mellonella L.)
ID Kopinšek, Tanja (Author), ID Kos, Katarina (Mentor) More about this mentor... This link opens in a new window

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Abstract
V raziskavi smo preučevali virulentnost entomopatogenih gliv iz rodov Beauveria in Metarhizium na ličinke mokarja in voščene vešče. Izolate gliv smo pridobili z izolacijo iz vzorcev tal različne rabe (trajni nasadi, njive s poljščinami, njive in vrtovi z vrtninami, travniki in gozd) v Sloveniji. V poskusu je bilo uporabljenih 14 izolatov 4 vrst entomopatogenih gliv iz rodov Beauveria in Metarhizium, in sicer dva izolata B. bassiana, en izolat M. guizhouense, pet izolatov M. robertsii in šest izolatov M. anisopliae. Ličinke voščene vešče in mokarja smo inokulirali s suspenzijo spor izolatov entomopatogenih gliv. Opazovanje je potekalo 16 dni. Rezultati smrtnosti so bili beleženi prvi, drugi, tretji, četrti, osmi, deveti, deseti, enajsti in šestnajsti dan po začetku poskusa. Ličinke voščene vešče so bile občutljivejše, zato je bila smrtnost pri večini izolatov večja kot pri ličinkah mokarja. Največjo smrtnost ličink so povzročili izolati T2 (100 %; 8. dan voščena vešča) in T7 (65 %; 16. dan mokar) pri glivi M. robertsii, T33 (60 % mokar; 10. dan) in T35 (100 % voščena vešča; 8. dan) pri vrsti M. anisopliae in T5 (100 % voščena vešča; 8. dan) pri glivi B. bassiana. Pri obeh testnih organizmih je bila največja smrtnost dosežena z izolatom Metarhizium robertsii T2 (voščena vešča 100 %; po 8. dneh, mokar 65 %; po 10. dneh).

Language:Slovenian
Keywords:entomopatogene glive, biotično varstvo rastlin, Beauveria bassiana, Metarhizium spp., Galleria mellonella, Tenebrio molitor
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Publisher:[T. Kopinšek]
Year:2020
PID:20.500.12556/RUL-119917 This link opens in a new window
UDC:632.937.14.01(043.2)
COBISS.SI-ID:30307843 This link opens in a new window
Publication date in RUL:13.09.2020
Views:717
Downloads:120
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Secondary language

Language:English
Title:Virulence testing of selected isolates of entomopathogenic fungi from the genus Beauveria in Metarhizium against Tenebrio molitor L. larvae and Galleria mellonella L. larvae
Abstract:
The efficacy entomopathogenic fungi from two common genera Beauveria and Metarhizium against the larve of greater wax moth and of the mealworm was tested in our research. We had isolates of fungal entomopathogens from different soil uses (permanent crops, fields with crops, fields and gardens with vegetables, meadows and forest) in Slovenia. In laboratory assay, we used 14 isolates of 4 species fungal entomopathogens from genus Beauveria and Metarhizium from those, two isolates Beauveria bassiana, one isolate Metarhizium guizhouense, five isolates Metarhizium robertsii and six isolates Metarhizium anisopliae. In the assay, we infected larvae of wax moth and mealworm. After inoculation assay lasted 16 days. We counted mycosed larvae one, two, three, four, eight, nine, eleven and sixteen days after inocualtion. Higher mortality was shown at more sensitive larvae of the greater wax moth than at the larvae of mealworm in most isolates. The highest morality was shown isolates T2 (100% at wax moth, day 8) and T7 (65% at mealworm, day 16) at fungus M. robertsii, T33 (60% at mealworm, day 10) and T35 (100% at wax moth, day 8) at fungus M. anisopliae and T5 (100% at wax moth, day 8) at fungus B. bassiana. Isolate of fungal entomopathogen Metarhizium robertsii T2 has shown the best results for both insect species (at wax moth 100% after eight days, at mealworm 65% after 10 days).

Keywords:entomopathogenic fungi, biological control, Beauveria bassiana, Metarhizium spp., Galleria mellonella, Tenebrio molitor

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