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Vloga dolgih nekodirajočih RNA pri osteoporozi
Müller, Eva (Author), Ostanek, Barbara (Mentor) More about this mentor... This link opens in a new window

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Abstract
Dolge nekodirajoče RNA (lncRNA) so transkripti, daljši od 200 nukleotidov, ki ne kodirajo proteinov. Posebno pozornost se jim v zadnjem času namenja v povezavi z osteoporozo – presnovno boleznijo kostnega tkiva, za katero je značilna zmanjšana trdnost kosti in za katero je obolelih več kot 200 milijonov ljudi na svetu. Z uporabo modernih molekularno genetskih tehnik (celične kulture, PCR, mikromreže, bioinformatika, imunoprecipitacija itd.) so določili že več lncRNA, med drugimi DANCR, HoxA-AS3, H19, MEG3, Linc-ROR, CRNDE, GAS5, MSC-AS1, AK077216 in MIRG, ki s svojim delovanjem vplivajo na kostno presnovo ter tako predstavljajo potencialne biološke označevalce in terapevtike/tarče za zdravljenje bolezni. Z uravnavanjem njihovega izražanja prek genske terapije ali RNA interference bi lahko omilili razvoj in posledice osteoporoze, vendar pa je študij na to temo zaenkrat malo in večina jih je potekala le v in vitro pogojih. Pred klinično uporabo lncRNA bo potrebnih še več študij spektra njihovega delovanja, medsebojnega vpliva in morebitnih stranskih učinkov pri človeku. Glavno oviro pri uporabi lncRNA kot bioloških označevalcev osteoporoze trenutno predstavlja pomanjkanje podatkov o njihovem izražanju med razvojem bolezni, glavno oviro pri uporabi lncRNA kot terapevtikov pa dejstvo, da še nimamo razvitih transportnih sistemov za vnos lncRNA (ali molekul, s katerimi bi njihovo delovanje uravnavali), v celice, ki sodelujejo v kostni presnovi.

Language:Slovenian
Keywords:nekodirajoče RNA, kostni metabolizem, osteoblasti, osteoklasti, osteoporoza
Work type:Bachelor thesis/paper (mb11)
Tipology:2.11 - Undergraduate Thesis
Organization:BF - Biotechnical Faculty
Year:2020
Publisher:[E. Müller]
UDC:606:616.71-007.234:601.4:577.218(043.2)
COBISS.SI-ID:31124227 This link opens in a new window
Views:95
Downloads:37
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Secondary language

Language:English
Title:The role of long non-coding rnas in osteoporosis
Abstract:
Long non-coding RNAs are transcripts with lengths exceeding 200 nucleotides that do not translate into proteins. Recently, they have been studied in the context of osteoporosis – a metabolic bone tissue disease, defined by lower bone density, which affects over 200 million people worldwide. Several lncRNAs, including DANCR, HoxA-AS3, H19, MEG3, Linc-ROR, CRNDE, GAS5, MSC-AS1, AK077216 and MIRG, involved in bone metabolism, and in osteoporosis, have already been identified using molecular genetic methods and tools (cell cultures, PCR, microarrays, immunoprecipitation, etc.). As such, they present potential biomarkers and therapeutic agents and/or targets. By regulating their expression using gene therapy or RNA silencing, modern medicine could delay the onset of osteoporosis and reduce the severity of its consequences. However, studies describing these lncRNAs are few and mostly done in in vitro conditions. Before any clinical application of lncRNAs, more studies will have to take place to properly assess the entirety of their functions, interactions, and possible side effects in humans. Currently, the main obstacle to using lncRNAs as biomarkers for osteoporosis is the lack of data describing their expression patterns during the development of the disease, while the main obstacle to using lncRNAs (or molecules for altering their function) as therapeutics is the lack of suitable delivery systems for their delivery into the cells of bone metabolism.

Keywords:non-coding RNA, bone metabolism, osteoblasts, osteoclasts, osteoporosis

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