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Uporaba tehnologije CRISPR-Cas9 v metabolnem inženiringu
ID Hrovatin, Matija (Author), ID Petrovič, Uroš (Mentor) More about this mentor... This link opens in a new window

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Abstract
Za proizvodnjo širokega spektra produktov se uporabljajo celične tovarne, ki lahko kot vir energije uporabljajo obnovljive vire in odpadke. Optimizacija delovanja celičnih tovarn je možna z metabolnim inženiringom. Ena od metod je sistem CRISPR-Cas9, ki učinkovito uvede želene spremembe v genom tarčnega organizma. Običajno s sistemom CRISPR-Cas9 izvedemo delecije, insercije ter spremembe tarčnih genov tako, da povečamo metabolni pretok skozi tarčne metabolne poti. Dobimo učinkovito celično tovarno, ki proizvaja velike količine tarčnega produkta. Tehnologijo CRISPR-Cas9 odlikuje visoka natančnost uvedbe sprememb, enostavnost, hitrost in nizka cena. V diplomski nalogi sem opisal aktualne uporabe tehnologije CRISPR-Cas9 za namen metabolnega inženiringa in izdelavo učinkovitih sevov mikroorganizmov, ki imajo velik potencial za uporabo v živilski, kozmetični in kemijski industriji ter v proizvodnji zdravil in biogoriv. Metabolni inženiring je ključnega pomena za nadaljnji razvoj in proizvodnjo produktov s celičnimi tovarnami.

Language:Slovenian
Keywords:metabolni inženiring, CRISPR-Cas9, celične tovarne, preurejanje genoma
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:BF - Biotechnical Faculty
Publisher:[M. Hrovatin]
Year:2020
PID:20.500.12556/RUL-119433 This link opens in a new window
UDC:606:62:602.6:578/579:601.4:577.21(043.2)
COBISS.SI-ID:31219715 This link opens in a new window
Publication date in RUL:09.09.2020
Views:1019
Downloads:149
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Secondary language

Language:English
Title:Use of CRISPR-Cas9 technology in metabolic engineering
Abstract:
Cell factories are applicable for production of a wide range of products from renewable sources and waste materials as an energy source. Optimization of cell factories operation conditions is further possible with metabolic engineering. One of the methods to achieve this is the CRISPR-Cas9 technology, which effectively introduces the desired changes to the genome of the target organism. Typically, deletions, insertions and other manipulations of target genes are performed with the CRISPR-Cas9 with the aim to increase metabolic flux through the target metabolic pathways. Using this approach, large quantities of the target product is synthesised through an environmentally benign process, via efficient cell factories. The advantages of CRISPR-Cas9 are high precision of genetic changes at targeted loci, simplicity, speed and economic efficiency of the process. In this thesis I will describe the state-of-the-art applications of CRISPR-Cas9 technology in metabolic engineering. I will also address production of efficient cell lines with potential applications in various fields such as food, cosmetic and chemical industry, production of biofuels and pharmaceuticals. Metabolic engineering is a key factor for the further development and manufacturing of desired products with cell factories.

Keywords:Metabolic engineering, CRISPR-Cas9, cell factories, genome modification

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