Neural differentiation of canine mesenchymal stem cells/multipotent mesenchymal stromal cells
ID Prpar Mihevc, Sonja (Author), ID Kokondoska Grgič, Vesna (Author), ID Kopitar, Andreja Nataša (Author), ID Mohorič, Luka (Author), ID Majdič, Gregor (Author)

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Background: The ability of adipose tissue-derived multipotent mesenchymal stromal cells/mesenchymal stem cells (ASCs) to differentiate in neural lineages promises progress in the field of regenerative medicine, especially for replacing neuronal tissue damaged by different neurological disorders. Reprogramming of ASCs can be induced by the growth medium with neurogenic inductors and specific growth factors. We investigated the neural differentiation potential of canine ASCs using several growth media (KEM, NIMa, NIMb, NIMc) containing various combinations of neurogenic inductors: B27 supplement, valproic acid, forskolin, N2-supplement, and retinoic acid. Cells were first preconditioned in the pre-differentiation neural induction medium (mitogenically stimulated; STIM1), followed by the induction of neuronal differentiation. Results: After 3, 6, and 9 days of neural induction, elongated neural-like cells with bipolar elongations were observed, and some oval cells with light nuclei appeared. The expression of neuronal markers tubulin beta III (TUBB3), neurofilament H (NF-H), microtubule-associated protein-2 (MAP2), and glial fibrillary acidic protein (GFAP) was observed using immunocytochemistry, which confirmed the differentiation into neurons and glial cells. Flow cytometry analysis showed high GFAP expression (between 70 and 90% of all cells) after cells had been growing three days in the neural induction medium a (NIMa). Around 25% of all cells also expressed adult neuronal markers NF-H and MAP2. After nine days of ASCs differentiation, the expression of all neural markers was reduced. There were no differences between the neural differentiation of ASCs isolated from female or male dogs. Conclusions: The differentiation repertoire of canine ASCs extends beyond mesodermal lineages. Using a defined neural induction medium, the canine ASCs differentiated into neural lineages and expressed markers of neuronal and glial cells, and also displayed the typical neuronal morphology. Differentiated ASCs can thus be a source of neural cellular lineages for the regenerative therapy of nerve damage and could be useful in the future for therapy or the modelling of neurodegenerative diseases.

Keywords:multipotent mesenchymal stromal cells, mesenchymal stem cells, dogs, differentiation, neurons, astrocytes
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:VF - Veterinary Faculty
MF - Faculty of Medicine
Publication status:Published
Publication version:Version of Record
Number of pages:12 str.
Numbering:Vol. 16, art. 282
PID:20.500.12556/RUL-118719 This link opens in a new window
ISSN on article:1746-6148
DOI:10.1186/s12917-020-02493-2 This link opens in a new window
COBISS.SI-ID:25006595 This link opens in a new window
Publication date in RUL:31.08.2020
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Record is a part of a journal

Title:BMC veterinary research
Publisher:Springer Nature
COBISS.SI-ID:515674393 This link opens in a new window


License:CC BY 4.0, Creative Commons Attribution 4.0 International
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:10.08.2020


Funder:EC - European Commission
Funding programme:European Regional Development Fund

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Project number:P4-0053
Name:Endokrini, imunski in encimski odzivi pri zdravih in bolnih živalih

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