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Uporaba rastlin za proizvodnjo biofarmacevtskih protiteles
Novak, Alenka (Author), Bohanec, Borut (Mentor) More about this mentor... This link opens in a new window

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Abstract
Monoklonska protitelesa, tako za terapevtske kot diagnostične namene, predstavljajo enega izmed najpomembnejših biofarmacevtskih izdelkov. Zaradi trenutnega in v prihodnje vedno večjega povpraševanja po njih je treba preiti na alternativne proizvodne platforme, ki bodo zmožne vsaj dopolniti že sedaj prekomerno preobremenjene tradicionalne proizvodne obrate. Ena od možnih alternativ je uporaba rastlinskih ekspresijskih sistemov. Ta tehnologija je že dokazala nekatere svoje prednosti v smislu varnosti, hitrosti, možnosti hitrega povečanja obsega, nižjih stroškov proizvodnje, visokih donosov, nadaljnje raziskave na področju glikoinženirstva pa posledično omogočajo celo izboljšanje biofarmacevtskih protiteles v smislu boljše stabilnosti in aktivnosti. Raznolikost rastlinskih sistemov (celične in tkivne kulture, uporaba vodnih rastlin, mahov, alg, celih rastlin) omogoča različno vrsto platform, ki lahko potencialno tekmujejo na različnih trgih, saj se prilagajajo konkretnim zahtevam. Ker je možnosti uporabe rastlin v ta namen veliko, sem se v diplomskem delu usmerila le na potencialno uporabo celih rastlin. Osredotočila sem se predvsem na načine transformacije (stabilna in prehodna), prednosti, pomanjkljivosti in težave, s katerimi se ta alternativa srečuje. Nekaj pozornosti sem namenila tudi pregledu trga in v kakšni meri bi lahko rastline doprinesle h globalnim potrebam po protitelesih. Na koncu sem podala še nekaj primerov, ki prikazujejo uspešno uporabo te tehnologije v večjem obsegu.

Language:Slovenian
Keywords:transformacija rastlin, transgene rastline, prehodni ekspresijski sistem, protitelesa, rastlinske platforme
Work type:Bachelor thesis/paper (mb11)
Tipology:2.11 - Undergraduate Thesis
Organization:BF - Biotechnical Faculty
Year:2020
Publisher:[A. Novak]
UDC:602.6:582.5/.9:606:616-097.3(043.2)
COBISS.SI-ID:27643651 This link opens in a new window
Views:264
Downloads:65
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Secondary language

Language:English
Title:Use of plant-based platform for production of biopharmaceutical antibodies
Abstract:
Monoclonal antibodies, both for therapeutic and diagnostic use, represent one of the most important biopharmaceutical products. Due to current and future increasing demand, there is an urgent need for alternative production platforms that can at least act as a complement to the overexploited mammalian fermentation systems. One possible alternative for the large-scale production of biopharmaceuticals is the use of plant expression systems. This technology has already proven its advantages in terms of safety, speed, potential for rapid scale-up production, lower production costs, high yields and the possibility to engineer a tailored antibody glycosylation profile, which allows us to produce even more stable and efficient antibodies. The diversity of plant systems (cell or tissue cultures, use of aquatic plants, mosses, algae, whole plants) allows adaptation to specific requirements of different platforms. Since the possibilities of use are broad I only focused on the potential use of whole plants. I described different methods of plant transformation (stable and transient expression), advantages, shortcomings, difficulties of this alternative platform, and the potential contribution of plant made antibodies to the global market. Finally, I have presented a few examples that have shown the successful use of plant-platforms on a larger scale.

Keywords:plant transformation, transgenic plants, transient expression, antibodies, plant platforms

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