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Medcelične interakcije bakterije Campylobacter jejuni
Šimunović, Katarina (Author), Smole Možina, Sonja (Mentor) More about this mentor... This link opens in a new window

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Abstract
Kampilobakterioza, katere najpogostejši povzročitelj je vrsta Campylobacter jejuni, je najpogosteje prijavljen bakterijski gastroenetritis v EU. Pomanjkanje ustreznih načinov za nadzor tega patogena kaže na veliko vrzel v razumevanju te bakterije; še posebej zanemarjeno področje so bakterijske interakcije C. jejuni. V tem delu smo raziskovali interakcije bakterije C. jejuni na več ravneh: (i) celično signaliziranje znotraj ene populacije, (ii) interakcije med različnimi sevi C. jejuni in (iii) medvrste interakcije med bakterijama Bacillus subtilis in C. jejuni. Pri proučevanju interakcij znotraj ene populacije smo ugotovili, da zaviranje celičnega signaliziranja vpliva na gibljivost, adhezijo na polistiren in invazijo bakterije C. jejuni v celice INT407. S testiranjem 20 rastlinskih pripravkov smo ugotovili, da ima izvleček rožnega korena (Sedum roseum) največji zaviralni učinek na celično signaliziranje. Pri proučevanju interakcij med sevi smo ugotovili, da so sevi C. jejuni sposobni sorodstvene diskriminacije. Manj sorodni sevi (98,00–99,13 % genetsko podobnostjo) so tvorili mejne linije med rojenjem na mehkem agarju. Sorodstvena diskriminacija je vplivala na koagregacijo sevov, med kokultivacijo različnih sevov pa smo ugotovili negativno interakcijo med sevoma tako pri rasti kot pri adheziji na polistiren. Pri raziskavi medvrstnih interakcij smo ugotovili, da interakcija bakterij B. subtilis in C. jejuni negativno vpliva na rast in preživetje bakterije C. jejuni v razmerah, ki omogočajo njeno aktivno rast. Največji zaviralni učinek smo določili pri B. subtilis PS-216. Iz naših ugotovitev sklepamo, da imajo bakterijske interakcije velik vpliv na socialno vedenje bakterije C. jejuni na vseh treh preiskovanih ravneh. Modifikacija teh interakcij lahko prispeva k nadzoru tega patogena v različnih okoljih.

Language:Slovenian
Keywords:Campylobacter jejuni, bakterijske interakcije, celično signaliziranje, sorodstvena diskriminacija, Bacillus subtilis, adhezija, invazija INT407, inhibicija rasti, gibljivost
Work type:Doctoral dissertation (mb31)
Tipology:2.08 - Doctoral Dissertation
Organization:BF - Biotechnical Faculty
Year:2020
Publisher:[K. Šimunović]
UDC:579.22/.26:579.6
COBISS.SI-ID:25025027 Link is opened in a new window
Views:125
Downloads:0
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Secondary language

Language:English
Title:Intercellular interactions of Campylobacter jejuni
Abstract:
Campylobacteriosis, most commonly caused by Campylobacter jejuni, is the most frequently reported bacterial gastroenteritis in the EU. The lack of adequate control measures for this pathogen exposes a major gap in the understanding of this bacterium. A particularly neglected area are bacterial interactions of C. jejuni. In this work we investigated the interactions of C. jejuni at different levels: (i) cell signaling within a population, (ii) interactions between C. jejuni strains, and (iii) inter-species interactions between Bacillus subtilis and C. jejuni. Within one population, we found that the inhibition of cell signaling affects motility, adhesion to polystyrene, and the invasion of C. jejuni into INT407 cells. By testing of 20 plant preparations we found that the strongest inhibitory effect was achieved by for the roseroot (Sedum roseum) extract. The study of C. jejuni inter-strain interactions reveled kin discrimination between C. jejuni strains. Less related strains (genetic similarity of 98.00 – 99.13 %) form a boundary line during swarming on soft agar. Kin discrimination affected the coaggregation of strains. The interaction of different strains was negative during co-cultivation and co-adhesion on polystyrene. The study of inter-species interactions revealed that the interaction between B. subtilis and C. jejuni negatively affects the growth and survival of C. jejuni in conditions that allow its growth. The strongest inhibitory effect was determined for B. subtilis PS-216. Our findings show that bacterial interactions have a strong effect on C. jejuni social interactions on all three studied levels. Modification of these interactions can contribute to the control of this pathogen in different environments.

Keywords:Campylobacter jejuni, bacterial interactions, cell signaling, kin discrimination, Bacillus subtilis, adhesion, invasion into INT407, growth inhibition, motility

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