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Analiza vsebnosti proantocianidinov v lesu bukve z UV-Vis Spektrofotometrom : diplomski projekt
ID Demšar, Davor (Author), ID Oven, Primož (Mentor) More about this mentor... This link opens in a new window, ID Poljanšek, Ida (Comentor), ID Petrič, Marko (Reviewer)

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Abstract
V tem delu smo raziskali vsebnost proantocianidinov v lesu bukve. Drevesa smo posekali v Kočevskem Rogu. Požagali smo jih tako, da smo dobili vsa potrebna tkiva za nadaljno raziskavo. Najprej smo izžagali kolute, ki smo jih nato razžagali, zmleli in posušili. Dobljene vzorce smo nato ekstrahirali, izvedli smo po dve ekstrakciji. Najprej smo izvedli ekstrakcijo po Soxhletu. Z njo smo dobili vsebnost vseh snovi, ki so topne v mešanici metanola in vode, ter vsebnost celokupnih hidrofilnih ekstraktivov. Nato smo izvedli še dietiletrsko ekstrakcijo, s katero smo dobili vsebnost lipofilnih ekstraktivov. Vsebnost proantocianidinov smo določili z UV-Vis spektrofotometrijo. Raziskava je pokazala, da imajo največjo vsebnost lipofilnih ekstraktivov vzorci poranitvenega dela, mrtve in žive grče. Najmanjšo vsebnost lipofilnih ekstraktivov smo izmerili v rdečem srcu. Največjo vsebnost celokupnih hidrofilnih ekstraktivov smo našli v mrtvi in živi grči. Najmanjšo vsebnost pa smo izmerili v rdečem srcu. Največjo vsebnost celokupnih ekstraktibilnih snovi smo našli v živi grči, najmanjšo v rdečem srcu. Največjo vsebnost proantocianidinov imajo vzorci mrtve grče, sledijo vzorci žive grče. Najmanjšo vsebnost pa imajo ponovno vzorci rdečega srca. V vseh primerih imajo vzorci rdečega srca najmanjšo vsebnost merjenih substanc.

Language:Slovenian
Keywords:bukev, Fagus sylvatica, ekstraktivi, proantocianidini, spektrofotometrija
Work type:Diploma project paper
Typology:2.11 - Undergraduate Thesis
Organization:BF - Biotechnical Faculty
Place of publishing:Ljubljana
Publisher:[D. Demšar]
Year:2015
Number of pages:VIII, 50 f.
PID:20.500.12556/RUL-117806 This link opens in a new window
UDC:630*813.2
COBISS.SI-ID:2457481 This link opens in a new window
Publication date in RUL:27.07.2020
Views:797
Downloads:119
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Secondary language

Language:English
Title:Analysis of proanthocyanidins content in beech wood using a UV-Vis spectrophotometer
Abstract:
In this study we researched the content of proanthocyanidins in beech wood. The trees were cut down in the Kočevski Rog forest. We cut down the trees in a way that enabled us to get all necessary tissues for further research. At first, we cut the reels, which were then sawn, milled and dried. Next, we extracted the resulting samples, we performed two extractions. Firstly we performed the Soxhlet extraction, which enabled us to measure the content of all substances soluble in mixture of methanol and water, and the content of hydrophilic extracts. Next, we performed the diethyl ether extraction. This method enabled us to trace the lipophilic extracts. The content of proanthocyanidins was measured using the UV-Vis spectrophotometry. Our research showed that the highest amount of lipophilic extracts can be found in samples of wounded wood tissue, dead and alive tree knots. The lowest amount of lipophilic extracts was tracked in the red heart. The highest amount of hydrophilic extracts was again found in dead and alive tree knots, while the lowest was again found in the red heart. The highest content of total extractible substances were found in live knots, the lowest, again in red heart. We traced the highest amount of proanthocyanidins in dead tree knots, followed by alive tree knots. The lowest content of proanthocyanidins was found in the red heart. In all cases the samples of the red heart had the lowest amount of traced substances

Keywords:beech, Fagus sylvatica, extractives, proanthocyanidins, spectrophotometry

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