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Vsebnost ekstraktivov v lesu robinije (Robinia pseudoacacia L.) : diplomski projekt
ID Vivod, Blaž (Author), ID Poljanšek, Ida (Mentor) More about this mentor... This link opens in a new window, ID Oven, Primož (Comentor), ID Petrič, Marko (Reviewer)

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Abstract
Analizirali smo variabilnost vsebnosti ekstraktivov in delež celokupnih fenolov v posameznih tkivih drevesa navadne robinije (Robinia pseudoacacia L.). Na območju Obreža (Središče ob Dravi, Slovenija) smo posekali drevesa robinije in iz njih izžagali vzorce. Iz vzorcev smo v laboratoriju izžagali posamezna tkiva, jih zmleli in pripravili za ekstrakcijo po Soxhletu. Ekstrakcijo smo najprej opravili z nepolarnim topilom cikloheksanom, da so se izločile lipofilne komponente. Nato smo hidrofilne komponente izločili s polarnim topilom acetonom/vodo. Acetonske vzorce ekstraktivov smo z UV/VIS spektroskopijo uporabili za določanje celokupnih fenolov. Največja povprečna vsebnost celokupnih ekstraktov je bila v jedrovini (8,41 %), najmanjša pa v beljavi (4,88 %). Največja povprečna vsebnost lipofilnih ekstraktivov (30,40 mg/g) je bila v območju skorje, medtem ko so imela ostala analizirana tkiva precej nižje vrednosti. V mrtvih grčah je bila povprečna vsebnost lipofilnih ekstraktivov najnižja (7,36 mg/g). Povprečna vsebnost hidrofilnih ekstraktivov je bila največja v jedrovini (78,26 mg/g), najmanjša je bila v območju beljave (25,20 mg/g). Največja povprečna vsebnost celokupnih fenolov je bila v območju jedrovine (34,89 mg/g), najmanjša pa v beljavi (4,70 mg/g). Periferni deli jedrovine so imeli največji delež celokupnih fenolov, proti centru stržena pa se je njihova vsebnost zmanjševala. Vsebnost celokupnih fenolov je bila pri vseh preučevanih drevesih navadne robinije večja v jedrovini kot v beljavi

Language:Slovenian
Keywords:Robinia pseudoacacia, ekstraktivi, fenoli, spektrofotometrija, grče, jedrovina
Work type:Diploma project paper
Typology:2.11 - Undergraduate Thesis
Organization:BF - Biotechnical Faculty
Place of publishing:Ljubljana
Publisher:[B. Vivod]
Year:2015
Number of pages:XIII, 56 f., [11] f. pril.
PID:20.500.12556/RUL-117676 This link opens in a new window
UDC:630*813
COBISS.SI-ID:2427273 This link opens in a new window
Publication date in RUL:20.07.2020
Views:1274
Downloads:145
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Secondary language

Language:English
Title:Contents of extractives in wood of black locust (Robinia pseudoacacia L.)
Abstract:
We analysed variability of extractive contents and the share of total phenols in different tissues of black locust trees (Robinia pseudoacacia L.). Black locust trees from Obrež (Središče ob Dravi, Slovenia) area were cut down and wood samples sawn out. Individual tissues werem sawn out, milled and prepared for extraction by Soxhlet. The extraction was conducted with a non-polar solvent cyclohexane for the lipophilic components, and with a polar solvent acetone/water for the hydrophilic components. Acetone extracts were used for the determination of total phenols done by UV/VIS spectroscopy. The highest average concentration of total extracts was found in heartwood (8.41 %), and the lowest in the sapwood (4.88 %). The bark area had the highest average content of lipophilic extractives (30.40 mg/g), while the other studied tissues had much lower values. The lowest average content of lipophilic extractives (7.36 mg/g) was in dead knots. The average content of hydrophilic extractives was the largest in the heartwood (78.26 mg/g) and the lowest in the range of sapwood (25.20 mg/g). The highest average concentration of total phenols was in the range of heartwood (34.89 mg/g), and the lowest in the sapwood (4.70 mg/g). Peripheral parts of heartwood had the largest share of total phenols, however, towards the centre of the core their value decreased. In all the researched robinia trees, the content of total phenols was greater in heartwood than in sapwood

Keywords:Robinia pseudoacacia, extractives, phenols, spectrophotometry, knots, heartwood

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