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Vpliv sevov bakterije Bacillus subtilis na transkripcijo genov imunskega odziva pri krompirju
Grubar, Teja (Author), Mandić Mulec, Ines (Mentor) More about this mentor... This link opens in a new window, Lukan, Tjaša (Co-mentor)

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Abstract
Rizobakterije, ki pozitivno vplivajo na rast rastlin (PGPR), izboljšajo rast rastlin in ugodno vplivajo na njihov imunski sistem. V magistrskem delu smo spremljali odziv rastlin krompirja na kolonizacijo bakterije Bacillus subtilis na transkriptomskem nivoju. V ta namen smo izbrali seva B. subtilis PS-216 in B. subitlis PS-218 ter rastline krompirja genotipov Rywal in Desiree. Spremljali smo kolonizacijo korenin s sevoma ter odziv v koreninah in listih rastlin v več časovnih točkah. Začetek tvorbe biofilma seva PS-218 smo na koreninah genotipa Rywal zaznali že po 30-minutni inkubaciji rastlin v predhodno namnoženi bakterijski kulturi, po dveh urah, je bil prisoten po celotni površini korenine. V primeru, ko bakterijska kultura ni bila predhodno namnožena, smo biofilm seva PS-216 na koreninah obeh genotipov zaznali po 6-urni inkubaciji, biofilm seva PS-218 po 5-urni inkubaciji. Tudi v nadaljnjih točkah inkubacije smo potrdili prisotnost biofilma na koreninah rastlin. Uspešno smo pokazali, da se rastline krompirja odzivajo na bakterijo B. subtilis. V listih in koreninah smo potrdili spremenjeno izražanje izbranih genov pri rastlinah inokuliranih z B. subtilis v vseh analiziranih časovnih točkah. Spremembe v izražanju genov v koreninah smo zaznali že v začetku nastajanja biofilma, odziv pa je bil najbolj izrazit po 2-urni inkubaciji. V listih inokuliranih rastlin je odziv na B. subtilis najbolj izrazit po prekonočni inkubaciji. Gena PTI5 in 13LOX sta bila izbrana kot markerska gena za spremljanje odziva rastlin krompirja na B. subtilis, saj je bilo njuno izražanje signifikantno inducirano v listih in koreninah rastlinah krompirja inokuliranih z B. subtilis.

Language:Slovenian
Keywords:krompir, Solanum tuberosum, imunski odziv rastlin, rizobakterije, PGPR, transkripcija genov, Bacillus subtilis, biofilm
Work type:Master's thesis/paper (mb22)
Tipology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Year:2020
Publisher:[T. Grubar]
UDC:579.26+579.64:577.2+575:635.21
COBISS.SI-ID:24069891 This link opens in a new window
Views:315
Downloads:9
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Secondary language

Language:English
Title:Effect of Bacillus subtilis strains on transcription of immune response genes in potato
Abstract:
Plant growth-promoting rhizobacteria (PGPR) exert beneficial effects on plant growth and immune system. In the MSc thesis, we monitored the response of potato plants post Bacillus subtilis root colonization on transcriptomic level. For this purpose, we selected two Bacillus subtilis strains, PS-216 and PS-218, and two potato genotypes Rywal and Desiree. We examined root colonization by B. subtilis strains as well as response in roots and leaves at different time points. The onset of PS-218 biofilm formation on the roots of Rywal genotype was observed after 30-minutes incubation period of plants in precultivated bacterial culture, after two hours, biofilm was observed along entire root surface. When the bacterial culture was not precultivated, PS-216 biofilm formation on roots of both genotypes was observed after 6-hours incubation period, and PS-218 biofilm after 5-hours of incubation. Also at all next examined time points, biofilm formation on roots was confirmed. We confirmed the response of potato plants post B. subtilis root treatment. We detected the altered expression of selected genes in leaves and roots of plants inoculated with B. subtilis at all analysed time points. We detected effect on gene expression in roots early at the time of biofilm formation, response was most pronounced after 2-hours incubation period. Response to B. subtilis in leaves was more pronounced after overnight incubation. PTI5 and 13LOX genes were identified as maker genes of potato response to B. subtilis, as their expression was significantly induced in leaves and roots of potato plants post B. subtilis inoculation.

Keywords:potato, Solanum tuberosum, plant immune response, rhizobacteria, PGPR, Bacillus subtilis, biofilm, gene transcription

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