Invasive mycoses are fungal infections that can cause a broad spectrum of clinical symptoms and diseases, which are dependant on the site of infection and the causative agent. Diagnostics of fungal pathogens in primary sterile bodily fluids is a key factor in treatment of patient. Sensitivity and time to detection are key factors of successfulness of test. Cultivation of primary sterile bodily fluids in hemoculture bottles is recommended, when there is a suspicion of invasive mycosis, by EFISG. For confirmation of invasive candidiasis, detection of pathogen with hemoculture method is golden standard. In our research we used simulated primary sterile bodily fluids, to assess sensitivity and time to detection of blood culture bottles for diagnostics of fungal pathogens. We found that sensitivity of BD BACTEC-Mycosis IC/F bottle for detection of pathogenic fungi in simulated primary sterile bodily fluids was 100 % and that sensitivity of BD BACTEC Plus Aerobic/F bottle was 95,3 %.We found that average time to detection of all groups of fungi, except some genera of moulds, is fastest when we used BD BACTEC-Mycosis IC/F bottle. Statistical difference in time to detection, in comparison with usage of BD BACTEC Plus Aerobic/F bottle, was noticed, when we detected Cryptococcus species at concentration of inoculum 10 CFU/mL and 100 CFU/mL (p = 0,002/0,001) and when we detected Aspergillus species at concentration of inoculum 100 CFU/mL (p = 0,027). We found out that usage of BD BACTEC Anaerobic Plus/F isn't suitable for detection of pathogenic fungi in primary sterile bodily fluids, for it's poor sensitivity (11,6 % - 13,95 %).
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