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Biotehnološki pristopi gojenja navadne lisičke Cantharellus cibarius.
ID Levstek, Lucija (Author), ID Petković, Hrvoje (Mentor) More about this mentor... This link opens in a new window, ID Avbelj, Martina (Co-mentor)

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Abstract
Lisičke so zaradi svoje unikatne mineralne, organske in strukturne kompozicije ena izmed najbolj zaželenih vrst gliv na svetu. Ker jih še ne znamo profitabilno gojiti, povpraševanje na trgu po njih pa je ogromno, se mnogo raziskav usmerja v razumevanje kompleksnega načina življenja lisičk in njihovo gojenje. Kljub mnogim raziskavam pa je večji del gojenja navadne lisičke Cantharellus cibarius še vedno uganka. Z naprednimi biotehnološkimi metodami in natančnim laboratorijskim delom so raziskovalci že uspeli izolirati čiste kulture različnih sevov lisičk s celega sveta in s tem omogočili dostopnost lisičk v genskih bankah po celem svetu. Prav tako so z uporabo biotehnologije uspeli modificirati trdna gojišča do te mere, da ustrezajo rasti micelija navadne lisičke, ki je zaradi odsotnosti simbiotske rastline na trdnem gojišču zelo težaven za gojenje in raste zelo počasi. Uspela je celo že indukcija mikorize s simbiotsko rastlino, ki pa je še precej slabo raziskana. Z uspehom indukcije mikorize se odpre tudi večje področje za nadaljnje raziskave, ki bi se lahko osredotočile na potek izmenjave snovi med glivo, rastlino in drugimi prisotnimi organizmi. Znanstveniki namreč sklepajo, da so v mikorizno izmenjavo rastline in glive vključene tudi mnoge različne vrste mikroorganizmov. Največjo oviro pri gojenju navadne lisičke Cantharellus cibarius pa predstavlja indukcija fruktifikacije. Ne glede na to, da so Danell in sod. (1997) in Ogawa in sod. (2019) že uspešno vzgojili gobe lisičke, pa nikomur še ni uspelo ugotoviti, kaj je faktor, ki inducira spolno razmnoževanje. V diplomskem delu so široko povzeta odkritja na področju gojenja lisičk in predstavljene omejitve, s katerimi se srečujejo znanstveniki po celem svetu.

Language:Slovenian
Keywords:lisičke, gojenje, mikroorganizmi, simbioza, ektomikoriza
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:BF - Biotechnical Faculty
Publisher:[L. Levstek]
Year:2020
PID:20.500.12556/RUL-117231 This link opens in a new window
UDC:601:582.28:579.64(043.2)
COBISS.SI-ID:21886979 This link opens in a new window
Publication date in RUL:03.07.2020
Views:1220
Downloads:204
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Secondary language

Language:English
Title:Biotechnological approaches of in vitro cultivation of the golden chantarelle Cantharellus cibarius
Abstract:
The golden chantarelle Chantarellus cibarius is one of the most desirable fungal species in the world because of its unique mineral, organic and structural composition. Since we do not know how to grow them profitably yet, and the market demand for them is enormous, much research is directed towards understanding the complex way of life of chantarelles and their cultivation. Despite much research, however, much of the cultivation of common chantarelle Cantharellus cibarius is still a puzzle. Through advanced biotechnology methods and precise laboratory work, researchers have already succeeded in isolating the pure cultures of various chantarelle strains from around the world, thus making them available in gene banks worldwide. By using biotechnology they have also been able to modify the solid media to the extent that it is effective for growth of the chantarelle mycelium, which is very difficult to grow on solid media because of the absence of the symbiotic plant. Even the induction of in vitro mycorrhiza with a symbiotic plant has succeeded, but it is still poorly understood. The success of mycorrhizal induction also opens up a larger area for further research that could focus on the course of metabolism between the fungus, plant, and other organisms present. Scientists conclude that many different types of microorganisms are involved in mycorrhizal exchange of plants and fungi. The major obstacle to the cultivation of the common chantarelle Cantharellus cibarius, however, is the induction of fructification. Notwithstanding that Danell et al. (1997) and Ogawa et al. (2019) have already successfully grown chantarelle mushrooms, but no one has yet been able to determine what is the factor that induces sexual reproduction. The diploma summarizes the findings in the field of chantarelle cultivation and presents the limitations faced by scientists around the world.

Keywords:chantarelle, cultivation, microorganisms, symbiosis, ectomycorrhiza

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