The purpose of this master's thesis was to test the effect that the biomolecular corona of chosen nanomaterials has on the interaction between them and the cells. We tested two groups of nanoparticles. For the first group, we incubated pristine nanoparticles in bovine serum albumin - BSA before testing, so a corona formed. For the second group we used nanoparticles of the same material without a preformed corona. We tested the effect of each group of nanoparticles on human neuroblastoma cells SH-SY5Y and tested seven different types of nanoparticles – SiO2, Ag, CuO, ZnO, and three different TiO2 nanoparticle samples. To asses the effect of the preformed protein corona versus no preformed corona on SH-SY5Y cells we used several cytotoxicity tests – resazurin test, neutral red uptake test (NR) and coomassie brilliant blue test (CBB). We also evaluated the amount of proteins bound to nanoparticles with a preformed corona using a BCA Protein Assay Test. We found that the ZnO and CuO nanoparticles with a preformed corona had a greater cytotoxic effect on cells, than those without a preformed corona. The cytotoxic effect of nanoparticles with and without the corona was simmilar in the case of Ag nanoparticles, and for the TiO2 ter SiO2 we did not notice significant cytotoxic effects. The effect of a preformed corona was more noticable in CuO and ZnO nanoparticles, which dissolute into ions. We presume that the differences in the effect of nanoparticles with and without a preformed corona are due to increased endocytosis of nanoparticles with a preformed corona, but this was not tested.
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