Validacija HPLC metode za selektivno določevanje kanabinoidov

Štefančič, Matevž (author), Pompe, Matevž (mentor)

Kanabinoidi oziroma fitokanabinoidi so organske aromatske molekule, ki jih najdemo na rastlini Konoplja. Za človeško farmakologijo so te molekule zanimive, saj se nanje odzivajo endokanabinoidni receptorji v telesu. Človeško telo namreč proizvaja telesu lastne endokanabinoide. Vedno bolj se jim pripisuje pozitivne, blagodejne in celo zdravilne učinke na človeško telo. V ta namen se je razvila in se še razvija potreba po analitskih tehnikah, ki dajejo verodostojne in točne rezultate V sklopu diplomskega dela smo preverili in validirali eno izmed analitskih tehnik določevanja kanabinoidov po International Council of Harmonisation (ICH) smernicah. High Performance Liquid Chromatography (HPLC) metoda za selektivno določanje kanabinoidov nam omogoča identifikacijo in kvantifikacijo kanabinoidov v številnih vzorcih, ki so ključni pri razvoju in raziskavah ter vplivih fitokanabinoidov na človeško telo.

• Language: Slovenian
• Keywords: High Performance Liquid Chromatography (HPLC) kromatografija, International Council of Harmonisation (ICH), Validacija metode, Fitokanabinoidi
• Work type: Bachelor thesis/paper
• Typology: 2.11 - Undergraduate Thesis
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Year: 2020
• PID: 20.500.12556/RUL-116799
• COBISS.SI-ID: 19588099
• Publication date in RUL: 11.06.2020

ŠTEFANČIČ, Matevž, 2020, Validacija HPLC metode za selektivno določevanje kanabinoidov [online]. Bachelor’s thesis. [Accessed 1 April 2025]. Retrieved from: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=eng&id=116799

Secondary language

• Language: English
• Title: Validation of the HPLC method for selective determination of cannabinoids

Abstract

Cannabinoids or Phytocannabinoids are organic aromatic molecules found in the Cannabis plant. These organic molecules are interesting for human pharmacology not only as the receptors response to them but also because our body is able to produce endocannabinoids of its own. They have been credited with having positive, beneficial and even healing effects. As the result of these properties, the need for analytical techniques that produce credible and accurate results has developed. This thesis examines one of the analytical techniques for determinating cannabinoids according to International Council of Harmonisation guidelines (ICH). The High Performance Liquid Chromatography method (HPLC) for the selective determination of cannabinoids enables us to identify and authentically quantify them in a number of different samples. Having a good analytical techinque is crucial in research, development and monitoring the effects of phytocannabinoids on the human body.

• Keywords: High Performance Liquid Chromatography (HPLC) kromatografija Chromatography, International Council of Harmonisation (ICH), Method validation, Phytocannabinoids