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Helicobacter labacensis sp. nov., Helicobacter mehlei sp. nov., and Helicobacter vulpis sp. nov., isolated from gastric mucosa of red foxes (Vulpes vulpes)
ID Gruntar, Igor (Author), ID Papić, Bojan (Author), ID Pate, Mateja (Author), ID Zajc, Urška (Author), ID Ocepek, Matjaž (Author), ID Kušar, Darja (Author)

URLURL - Source URL, Visit https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004050 This link opens in a new window

Abstract
Six Helicobacter- like isolates were recovered from 15 gastric mucosa samples of red foxes (Vulpes vulpes) shot by hunters in the surroundings of Ljubljana, Slovenia. Gram- negative, tightly coiled, intensely motile, 7–15 μm long and ≤1 μm wide bacteria grew on the biphasic blood agar plates. By using a genus- specific polymerase chain reaction (PCR), all isolates were confirmed as Helicobacter sp. and subsequently subjected to whole- genome sequencing (WGS). Five isolates showed a genome-wide average nucleotide identity (ANI) value of <95 % to the previously described Helicobacter species and one isolate was classified as Helicobacter felis. In the five unidentified isolates, the 16S rRNA gene sequence similarity to the type strains of all Helico-bacter species ranged from 98.6 to 98.9 %. Their taxonomic status was established using a polyphasic taxonomic approach comprising the core genome- based phylogeny, morphological and phenotypic characteristics, including an analysis of matrix- assisted laser desorption/ionisation time- of- flight (MALDI-TOF) mass spectra. Phylogeny revealed the existence of three novel and well- supported clusters, with Helicobacter bizzozeronii and Helicobacter baculiformis being the most closely related species. The isolates also differed from the previously described species in their MALDI-TOF profiles and some biochemical characteris-tics. In conclusion, the data presented herein indicate that the obtained isolates, excluding H. felis isolate, represent three novel Helicobacter species, for which the names Helicobacter labacensis sp. nov., Helicobacter mehlei sp. nov., and Helicobacter vulpissp. nov. are proposed, with isolates L9T (=DSM 108823T=CRBIP 111719T), L15T (=DSM 108730T=CCUG 72910T) and L2T (=DSM 108727T=CCUG 72909T) as type strains, respectively

Language:English
Keywords:Helicobacter, novel species, red fox, Vulpes vulpes, gastric mucosa
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:VF - Veterinary Faculty
Publication status:Published
Publication version:Version of Record
Year:2020
Number of pages:Str. 2395-2404
Numbering:Vol. 70, Iss. 4
PID:20.500.12556/RUL-116676 This link opens in a new window
UDC:636.09:579
ISSN on article:1466-5026
DOI:10.1099/ijsem.0.004050 This link opens in a new window
COBISS.SI-ID:5030010 This link opens in a new window
Publication date in RUL:02.06.2020
Views:1158
Downloads:133
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Record is a part of a journal

Title:International journal of systematic and evolutionary microbiology
Shortened title:Int J Syst Evol Microbiol
Publisher:Society for General Microbiology
ISSN:1466-5026
COBISS.SI-ID:2145556 This link opens in a new window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:02.06.2020

Projects

Funder:ARRS - Slovenian Research Agency
Project number:P4-0092
Name:Zdravje živali, okolje in varna hrana

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