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The interaction between the osteosarcoma cell and stainless steel surface, modified by high-fluence, nanosecond laser pulses
Hočevar, Matej (Author), Šetina, Barbara (Author), Godec, Matjaž (Author), Kononenko, Veno (Author), Drobne, Damjana (Author), Gregorčič, Peter (Author)

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Abstract
The irradiation of metallic surfaces by high-fluence laser pulses in an oxygen-containing atmosphere inevitably modifies the surface topography, chemistry, and wettability. These modifications significantly influence cell-surface interactions and, consequently, surface biocompatibility. We investigate how surface texturing by high-fluence nanosecond laser pulses from a Nd:YAG laser (wavelength of 1064 nm) influences cell adhesion and morphology with the aim of assessing its impact on initial cell behaviour. Quantitative and qualitative analysis of osteosarcoma cell adhesion, viability, and cell morphology were evaluated after 24-hour exposure to non-treated and laser-textured stainless-steel (AISI 316L) surfaces by fluorescent and scanning electron microscopy. The results reveal that this, initial interaction between the cells and the laser-textured surfaces leads to round shaped cells with a smaller footprint. Contrarily, on the non-processed stainless-steel and control-glass surfaces the polygonal, highly elongated, and flattened cells are observed. The cells on the laser-textured surfaces are less dendritic, with short tubular protrusions and an overexpression of extracellular vesicles, which are rarely found on non-treated and control samples. This likely happens due to the formation of nanostructured, high-temperature oxides that are induced by laser ablation. The analysis by X-ray photoelectron spectroscopy reveals that the laser-textured stainless-steel surfaces contain Cr hexavalent oxide, which is more toxic than the native oxide layer on the non-processed samples.

Language:English
Keywords:laser surface engineering, laser processing, biocompatibility, biological cell, nanosecond-laser ablation, laser texturing, MG63 cells, cell adhesion
Work type:Article (dk_c)
Tipology:1.01 - Original Scientific Article
Organization:BF - Biotechnical Faculty
FS - Faculty of Mechanical Engineering
Year:2020
Number of pages:12 str.
Numbering:Vol. 394, art. 125878
UDC:621.7.015(045)
ISSN on article:0257-8972
DOI:10.1016/j.surfcoat.2020.125878 This link opens in a new window
COBISS.SI-ID:14290947 This link opens in a new window
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Downloads:427
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Record is a part of a journal

Title:Surface & coatings technology
Shortened title:Surf. coat. technol.
Publisher:Elsevier Sequoia
ISSN:0257-8972
COBISS.SI-ID:3084815 This link opens in a new window

Document is financed by a project

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Project no.:P2-0392
Name:Optodinamika

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Project no.:P1-0207
Name:Toksini in biomembrane

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Project no.:P2-0132
Name:Fizika in kemija površin kovinskih materialov

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Project no.:Z2-9215
Name:Razvoj biomaterialov z laserskim teksturiranjem kovinskih zlitin (BiomatLasTex)

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Project no.:J2-1741
Name:Lasersko mikro in nanostrukturiranje za razvoj biomimetičnih kovinskih površin z edinstvenimi lastnostmi (LaMiNaS)

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Project no.:J1-9162
Name:Neurotoksičnost ali neuroprotektivnost nanomatrialov: vpliv biokorone

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Project no.:J2-1729
Name:Nova biorazgradljiva Fe-Mn zlitina izdelana z konvencionalnim postopkom in s postopkom dodajnih tehnologij s prilagojeno biorazgradljivostjo

Funder:Drugi - Drug financer ali več financerjev
Funding Programme:Ministry of Education, Science and Sport of the Republic Slovenia
Project no.:OP20.03531
Name:Modeliranje termomehanskega procesiranja aluminijevih zlitin za vrhunske izdelke
Acronym:MARTIN

Funder:EC - European Commission
Funding Programme:European Regional Development Fund
Project no.:OP20.03531
Name:Modeliranje termomehanskega procesiranja aluminijevih zlitin za vrhunske izdelke
Acronym:MARTIN

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:06.05.2020

Secondary language

Language:Slovenian
Keywords:laserski inženiring površin, laserske obdelave, biokompatibilnost, biološke celice, nanosekundno-laserska ablacija, lasersko teksturiranje, celice MG63, adhezija celic

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