izpis_h1_title_alt

Sestava in aktivnost mikrobne združbe nitrifikacijskih bakterij v imobilizirani biokulturi : magistrsko delo
Brajer Humar, Barbara (Author), Mandić-Mulec, Ines (Mentor) More about this mentor... This link opens in a new window

.pdfPDF - Presentation file, Download (1,79 MB)
MD5: 23ECD417EBBEC26E4162B90FE0D2989F

Abstract
V procesu čiščenja odpadne vode na čistilnih napravah je proces nitrifikacije ključen za odstranjevanje enega od najpomembnejših hranil-dušika- z nitrifikacijskimi bakterijami. Spremljali smo proces nitrifikacije ter prisotnost, pestrost in stabilnost mikrobnih združb na osnovi zaporedij 16S rRNA in specifičnih funkcionalnih genov (npr. amoA) v industrijskih in laboratorijskih reaktorjih z imobilizirano biokulturo. Zanimalo nas je predvsem kako se združba nitrifikacijskih bakterij v imobilizirani biokulturi ali biofilmu na prosto plavajočih plastičnih nosilnih elementih Kaldnes v biofilmu odziva glede na različno kvaliteto odpadne vode ter letni čas. Mikrobno združbo na CČN Domžale-Kamnik (CČN DK) smo analizirali z metodo TRFLP in s sekvenciranjem genov za 16S rRNA in genov amoA. Z analizo TRFLP smo ugotovili, da se je, kljub nihanju strukture združbe v času, vzpostavila določena razlika med vzorci iz pilotnih sistemov z mehansko čiščeno in sintetično odpadno vodo, sama velikost (industrijski vs. mini reaktor) sistema pa ni bila pomembna. Še bolj očitna razlika med združbami, ki so bile izpostavljene različnim odpadnim vodam, se je pokazala z analizo funkcionalnega nitrifikacijskega gena amoA. In sicer so se zunanji industrijski reaktor in mini pilotni reaktorji, obremenjeni z mehansko čiščeno odpadno vodo, razlikovali tako po sestavi kot po pestrosti nitrifikacijskih mikroorganizmov. Pestrost splošne 16S rRNA mikrobne združbe je bila višja v reaktorjih z mehansko čiščeno odpadno vodo, medtem ko so reaktorji z sintetično odpadno vodo imeli večjo pestrost nitrifikacijske (amoA) mikrobne združbe. Na podlagi rezultatov sklepamo, da ima kvaliteta odpadne vode večji vpliv na sestavo združbe kot pa dejavniki okolja kot sta temperatura ali pa velikost reaktorjev.

Language:Slovenian
Keywords:mikrobna združba, nitrifikacija, imobilizirana biokultura, bioreaktor, nitrifikacijske bakterije, čiščenje odpadnih vod
Work type:Master's thesis (m2)
Tipology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Year:2016
Publisher:[B. Brajer Humar]
Number of pages:IX, 63, [6] f.
UDC:579:628.3:631.461.2(043.2)=163.6
COBISS.SI-ID:860023 This link opens in a new window
Views:275
Downloads:114
Metadata:XML RDF-CHPDL DC-XML DC-RDF
 
Average score:(0 votes)
Your score:Voting is allowed only to logged in users.
:
Share:AddThis
AddThis uses cookies that require your consent. Edit consent...

Secondary language

Language:English
Title:Structure and diversity of nitrifying microbial community in biofilm
Abstract:
In the process of treating waste water in biological treatment plants, the nitrification process is crucial for the removal of one of the most important nutrients, i.e. nitrogen, using nitrification bacteria. The aim of our work was to produce a study of the process of nitrification itself, including an analysis of the presence, diversity and stability of microbial communities based on the 16S rRNA sequences and specific functional genes (eg. amoA) in industrial-scale and laboratory-scale reactors with immobilized bioculture. Industrial-scale reactors treat wastewater of temporary variable composition under different weather conditions, while the microbial population of wastewater treatment plants are often studied in controlled laboratory-scale systems with defined influent at a constant temperature. 16S rRNA and ammonia oxidising amoA-gene-defined bacterial community structure was investigated in industrial-scale and laboratory-scale moving bes biofilm reactor (MBBR) treating municipial wastewater or synthetic ammonium solition. Nitrification activity, 16S rRNA and amoA gene TRFLP profiles were comparable between industrial and laboratory-scale reactors with municipial wastewater. Reactors with synthetic ammonium solution exhibited higher nitrification and higher relative abundance of Nitrosomonadaceae and Nitrospiraceae families but only small changes in general bacterial community structure was detected compared to MBBR reactors treating municipial wastewater. Nitrosomonas europea lineage dominated in reactors treating municipial wastewater, whilu uncultivated Nitrosomonas-like sequences prevailed in reactors with synthetic ammonia solution. These results suggest that influent type has a stronger influence on community structure than operational conditions, such as temperature or reactor size.

Keywords:microbial community, nitrification, biofilm, bioreactor, nitrifying bacteria, wastewater treatment

Similar documents

Similar works from RUL:
Similar works from other Slovenian collections:

Comments

Leave comment

You have to log in to leave a comment.

Comments (0)
0 - 0 / 0
 
There are no comments!

Back