Differences between differently vital groups of pedunculate oaks (Quercus robur) were determined in diameter increment and wood-anatomical features. Ring-width and latewood chronologies were standardized using modified negative exponential curve. Large-scale drainage manipulation in 1982 in forest stand from Cigonca first caused changes in ring width, latewood and parameter "total vessel area". Not until the year 1995 signs of dieback are also visible in smaller sizes of conductive elements. "Strongly Declining" group from Cigonca is responsive only to the mean monthly and seasonal temperatures. Dependence is strictly negative - for dendrochronological and wood-anatomical parameters. "Vital" and "Declining" groups from Mlače are responsive to the mean temperatures and the sum of precipitations. Wood-anatomical parameters from "Vital" group from Mlače showed higher responsive rate to temperatures than dendrochronological parameters, which are mostly responding to June precipitation. Higher winter and spring precipitation cause smaller conductive elements but do not affect the "total vessel area". Parameter "maximal vessel area" was recognized as a potential parameter for reconstruction of mean temperatures from March to June.