Vpliv gliv na razkroj velikih lesnih ostankov bukve v pragozdnem rezervatu Rajhenavski Rog : diplomsko delo - univerzitetni študij - 1. stopnja

Finžgar, Domen (author), Kraigher, Hojka (mentor)

Ugotavljanje vpliva gliv na razgradnjo velikih lesnih ostankov (VLO) v pragozdnem rezervatu smo razčlenili na dva dela. Prvi se je ukvarjal z ugotavljanjem zmanjševanja gostot VLO s stopnjo razgradnje, drugi pa z opisom pojavnosti gliv v vzorcih VLO z metodo gelske elektroforeze v gradientu denaturantov (DGGE). Z namenom ugotavljanja zanesljivosti drugačnih, zdravju varnejših metod od živosrebrne metode, tudi na vodnem volumometru. Pri tem je bila pilotno uporabljena tehtnica Sartorius MSA 323S z dodatkom za merjenje gostot YDK01. Merili smo osnovne, nasičene in absolutne gostote. Po pričakovanjih sta nasičeni in osnovni gostoti pokazali trend padanja gostot. Tehtnica Sartorius se je izkazala za zanesljivo in hitro metodo merjenja gostot v vodi, ki pa jo bo potrebno še optimizirati. Molekularne metode ugotavljanja prisotnosti gliv v VLO so v Sloveniji v razvoju. Na podlagi ekstrakcije glivne DNA iz vzorcev VLO smo s pomočjo analitske metode DGGE uspeli pridobiti profile združb gliv za posamezni vzorec. Rezultati so pokazali, da so nekatere glive značilne za začetne stopnje razkroja, druge pa za končne stopnje. Pestrost profilov se s stopnjo razkroja veča, odstopa le stopnja razkroja 1, ki ima nepričakovano veliko stopnjo pestrosti. Razvrstitev vzorcev glede na podobnost DGGE profilov ni bila skladna z razvrstitvijo v stopnje razgradnje, variabilnosti med vzorci znotraj stopenj razgradnje je bila visoka. Povezave med genetskimi in geografskimi razdaljami niso bile ugotovljene.

• Language: Slovenian
• Keywords: bukev, DGGE, gostota, glive, Rajhenav, VLO, voda, živo srebro
• Work type: Bachelor thesis/paper
• Typology: 2.11 - Undergraduate Thesis
• Organization: BF - Biotechnical Faculty
• Place of publishing: Ljubljana
• Publisher: [D. Finžgar]
• Year: 2013
• Number of pages: XII, 48 str. + pril.
• PID: 20.500.12556/RUL-115133
• UDC: 630*1
• COBISS.SI-ID: 3715750
• Publication date in RUL: 14.04.2020

Secondary language

• Language: English
• Title: The role of fungi in the decay of coarse woody debris of European Beech (Fagus Sylvatica L.) in the forest reserve Rajhenavski Rog

Abstract

Researching the role of fungi in the decay of coarse woody debris (CWD) of European beech was divided in two parts. Determining the loss of CWD density during decay was done in part one, while the second part investigated the distribution and succession of fungi in the decaying CWD, using the denaturating gradient gel electophoresis (DGGE). To study the suitability of other, less toxic, density measuring techniques than the standard mercury one,samples were measured in a mercury medium as well as in a water medium. For water measurements we used Sartorius MSA 323S scale with YDK01 Density Determination Kit for the first time in our labs. We measured basic, absolute and saturated densities. Lower densities in higher decay phases were detected,as expected. The YDK01 Density Determination Kit proved to be a reliable method of measuring wood sample densities in water. However, some optimizations will have to be considered in the future. Molecular methods for studying fungi in CWD are in Slovenia still in early phases. Based on DNA extracted from fungi inside CWD, we conducted a DGGE analysis, resulting in fungi community profiles for each sample. According to the results, different decay stages host different types of fungi species. The number of fungi species per sample increases with higher decay phases. Genetic distances determined from DGGE profiles and geographic distances were not in high correlation.