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Primerjava prehranskih dopolnil iz alg: antioksidativni potencial in izotopska sestava
Kejžar, Jan (Author), Poklar Ulrih, Nataša (Mentor) More about this mentor... This link opens in a new window, Ogrinc, Nives (Co-mentor)

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Abstract
Antioksidativni potencial in izvor prehranskih dopolnil na osnovi alg sta slabo znana, saj prehranska dopolnila nimajo stroge regulacije tako kot zdravila in živila. Z določanjem antioksidativnega potenciala - AOP (metoda DPPH in Folin-Ciocalteu), elementne sestave (XRF in ICP-MS) in izotopske sestave lahkih elementov (EAIRMS), smo medsebojno ovrednotili posamezne vzorce prehranskih dopolnil na osnovi Aphanizomenon flos-aquae - AFA, Spirulina sp., Chlorella sp. in rjavih alg. AFA imajo 4,4 krat višji AOP v primerjavi s Chlorella sp. in 2,7 krat višji v primerjavi s Spirulina sp., medtem ko imajo rjave alge zanemarljiv AOP. Vsebnosti elementov v priporočenih odmerkih PD ne presegajo mejnih vrednosti za vnos esencialnih elementov. Koncentracija toksičnih elementov (arzen, kadmij, živo srebro in svinec) v vzorcih je nizka in posledično ne predstavlja tveganja za zdravje potrošnikov. Nekateri vzorci imajo bistveno povišano vsebnost silicija, kar nakazuje na dodatek nedeklariranega SiO2. S pomočjo statistične obdelave (analiza glavnih komponent in analiza razvrščanja enot v skupine) rezultatov analiz, lahko potrdimo enoten izvor AFA, ki naj bi bilo jezero Klamath. Havajsko S. pacifica je tudi možno ločiti od ostalih vzorcev predvsem zaradi značilne elementne sestave. Zaradi različnih izotopskih razmerij stabilnih elementov (C, N in S) v vzorcih Spirulina sp. in Chlorella sp. predvidevamo, da proizvajalci uporabljajo hranila različnega izvora in različne tehnike gojenja alg.

Language:Slovenian
Keywords:prehrana, prehranska dopolnila, alge, Spirulina sp., Chlorella sp., klamatske alge, rjave alge, elementna sestava, izotopska sestava, antioksidativni potencial, fenolne spojine, analitske metode, DPPH, Folin-Ciocalteu
Work type:Master's thesis/paper (mb22)
Tipology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Year:2020
Publisher:[J. Kejžar]
UDC:582.232:641:665.939.35:543.51
COBISS.SI-ID:5180792 Link is opened in a new window
Views:163
Downloads:73
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Secondary language

Language:English
Title:Comparison of algae dietary supplements: antioxidative potencial and izotopic composition
Abstract:
Antioxidative potential and origin of algae based food supplements are relatively unknown, as food supplements do not have strict regulation compared to food and medicinal drugs. Our goal was to evaluate different samples of food supplements that are based on Aphanizomenon flos-aquae – AFA, Spirulina sp., Chlorella sp. and brown algae, by determining antioxidative potencial - AOP (methods DPPH and Folin-Ciocalteu), elemental composition analysis (XRF and ICP-MS) and ratios of stables isotopes of light elements (C, N and S; EA-IRMS method). AFA samples have 4.4 higher AOP compared to Chlorella sp. and 2,7 higher compared to Spirulina sp., while AOP in brown algae is negligible. Manufacturer's suggested doses of samples do not exceed upper intake level of essencial elements. Concentrations of toxic elements (arsenic, cadmium, mercury and lead) are low and do not represent risk to consumer's health. Few samples have increased silicon content, which could mean addition of undeclared additive SiO2. Statistical analysis (principal component analysis and cluster analysis) has confirmed that AFA samples originate from the same source, which is supposedly lake Klamath. Hawaiian S. pacifica can also be differentiated from other samples, due to characteristic elemental composition. Different isotopic ratios of stable elements (C, N and S) of Spirulina sp. and Chlorella sp. samples are consequence of using different nutrient sources and algae growing techniques.

Keywords:food, food supplements, algae, Spirulina sp., Chlorella sp., Klamath algae, kelp, elemental composition, isotopic composition, antioxidative potential, phenolic compounds, analytical methods, DPPH, Folin-Ciocalteu

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