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Stress reshapes the physiological response of halophile fungi to salinity
ID
Pérez-Llano, Yordanis
(
Author
),
ID
Caridad Rodríguez-Pupo, Eya
(
Author
),
ID
Druzhinina, Irina S.
(
Author
),
ID
Chenthamara, Komal
(
Author
),
ID
Cai, Feng
(
Author
),
ID
Gunde-Cimerman, Nina
(
Author
),
ID
Zalar, Polona
(
Author
),
ID
Gostinčar, Cene
(
Author
),
ID
Kostanjšek, Rok
(
Author
),
ID
Folch-Mallol, Jorge Luis
(
Author
),
ID
Batista-García, Ramón Alberto
(
Author
),
ID
Rayo Sánchez-Carbente, María del
(
Author
)
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https://www.mdpi.com/2073-4409/9/3/525
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Abstract
(1) Background: Mechanisms of cellular and molecular adaptation of fungi to salinity have been commonly drawn from halotolerant strains and few studies in basidiomycete fungi. These studies have been conducted in settings where cells are subjected to stress, either hypo- or hyperosmotic, which can be a confounding factor in describing physiological mechanisms related to salinity. (2) Methods: We have studied transcriptomic changes in Aspergillus sydowii, a halophilic species, when growing in three different salinity conditions (No NaCl, 0.5 M, and 2.0 M NaCl). (3) Results: In this fungus, major physiological modifications occur under high salinity (2.0 M NaCl) and not when cultured under optimal conditions (0.5 M NaCl), suggesting that most of the mechanisms described for halophilic growth are a consequence of saline stress response and not an adaptation to saline conditions. Cell wall modifications occur exclusively at extreme salinity, with an increase in cell wall thickness and lamellar structure, which seem to involve a decrease in chitin content and an augmented content of alfa and beta-glucans. Additionally, three hydrophobin genes were differentially expressed under hypo- or hyperosmotic stress but not when the fungus grows optimally. Regarding compatible solutes, glycerol is the main compound accumulated in salt stress conditions, whereas trehalose is accumulated in the absence of salt. (4) Conclusions: Physiological responses to salinity vary greatly between optimal and high salt concentrations and are not a simple graded effect as the salt concentration increases. Our results highlight the influence of stress in reshaping the response of extremophiles to environmental challenges.
Language:
English
Keywords:
Aspergillus sydowii
,
genome
,
fungal cell wall
,
compatible solutes
,
halophilic fungi
,
hydrophobins
,
fungal transcriptomics
,
osmotic stress
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
BF - Biotechnical Faculty
Publication status:
Published
Publication version:
Version of Record
Year:
2020
Number of pages:
21 str.
Numbering:
Vol. 9, iss. 3, art. 525
PID:
20.500.12556/RUL-114832
UDC:
579
ISSN on article:
2073-4409
DOI:
10.3390/cells9030525
COBISS.SI-ID:
5325903
Publication date in RUL:
17.03.2020
Views:
1396
Downloads:
345
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Record is a part of a journal
Title:
Cells
Shortened title:
Cells
Publisher:
MDPI
ISSN:
2073-4409
COBISS.SI-ID:
519958809
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:
01.03.2020
Projects
Funder:
Other - Other funder or multiple funders
Funding programme:
Consejo Nacional de Ciencia y Tecnología
Project number:
CB 250831
Funder:
Other - Other funder or multiple funders
Funding programme:
Consejo Nacional de Ciencia y Tecnología
Project number:
CB 285816
Funder:
Other - Other funder or multiple funders
Funding programme:
PRODEP
Project number:
UAEMOR-PTC-333
Funder:
Other - Other funder or multiple funders
Funding programme:
Consejo Nacional de Ciencia y Tecnología, Scholarship support
Funder:
FWF - Austrian Science Fund
Project number:
P25613-B20
Funder:
FWF - Austrian Science Fund
Project number:
P25745-B20
Funder:
Other - Other funder or multiple funders
Funding programme:
Vienna Science and Technology Fund (WWTF)
Project number:
LS13-048
Funder:
ARRS - Slovenian Research Agency
Project number:
I0-0021
Name:
Infrastrukturni center za raziskave molekulskih interakcij, Razvojno raziskovalni center za proučevanje rasti in razvoja kmetijskih rastlin, IC Mycosmo
Funder:
ARRS - Slovenian Research Agency
Project number:
I0-0022
Name:
Mreža raziskovalnih infrastrukturnih centrov Univerze v Ljubljani (MRIC UL)
Funder:
ARRS - Slovenian Research Agency
Project number:
P1-0170
Name:
Molekulski mehanizmi uravnavanja celičnih procesov v povezavi z nekaterimi boleznimi pri človeku
Funder:
ARRS - Slovenian Research Agency
Project number:
P1-0198
Name:
Molekularno-biološke raziskave mikroorganizmov
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