The assessment instruments for patients with multiple sclerosis are numerous due to variability in symptoms. They can be performed as single assessment tool or in combination. The article reviews the most common functional tests for measuring the level of motor disability in multiple sclerosis patients. Impairment of muscle function can be assessed using isometric, isokinetic and hand held dynamometry. For functional assessment of hand function Nine-Hole Peg Test, Action Research Arm Test, Jebsen’s Hand Functional Test and Upper Extremity Evaluation Performance Test are used. Berg balance scale and Functional Reach test were used in most clinical research for balance assessment. Walking ability was assessed by 10-m Walk Test, Fatigue Index during walking, observational video analysis and by using pedometer. The level of respiratory dysfunction in patients with multiple sclerosis is evaluated by measuring Maximal Voluntary Cough, Maximal Expiratory and Inspiratory Pressure. For measuring the frequency and severity of bladder and bowel symptoms the use of different diaries were recommended. The use of different measurement tools for assessment the level of motor dysfunction in multiple sclerosis patients is based on measurement tools’ characteristics. It depends on the purpose and appropriate addressed question what measurement tool or combination of measurement tools will be used.